表达序列标签研究进展及其在甲壳动物中的应用概况

Research Advance of Expressed Sequence Tag(EST) and Its Application in Crustacean

随着生物信息学的发展,表达序列标签(EST)在分子标记开发、新基因分离鉴定、基因表达谱分析、基因组功能注释、基因电子克隆等方面具有重要作用。简要介绍了EST分析的原理及其在基因识别、基因预测、物理图谱的构建、DNA芯片制备等方面的应用概况。综述了甲壳动物EST的研究现状,并对EST的应用前景进行了展望。

With the development of bioinformatics,expressed sequence tag（EST） played an important role in molecular markers development,new genes isolation and identification,gene expression profile analysis,genome functional annotation and silico gene cloning.The principle of EST analysis and its applications in gene identification,gene prediction,physical map construction and DNA chip preparation was briefly introduced.In addition,the research status of crustacean EST and the prospect of EST application were also summarized.