摘要
采用紫外线诱变处理与含药(百菌清)培养基驯化相结合的方法,对拮抗木霉菌株T32进行改良,获得了4株在百菌清杀菌剂2 000 mg/L浓度水平下仍能较好生长的抗性菌株,并通过测定其生长速率、产孢量、敏感性、拮抗作用以及遗传稳定性等指标,筛选出了性状优良的抗性菌株T32-5-10m。试验结果表明,经诱变的木霉菌株T32-5-10m与亲本木霉菌株T32相比较,其抗药性提高了20倍,生长速率和产孢能力均高于亲本菌株,连续转接10代,其抗药性十分稳定,且对供试3种土传病原真菌的抑制率达87.56%～89.11%。
Antagonistic Trichoderma strain T32 was improved by ultraviolet mutation and chlorothalonil medicinal cultivation.4 chlorothalonil resistance strains which could grow well when chlorothalonil concentration was 2 000 mg/L were obtained.A resistance strain T32-5-10m with good features was screened by testing the growth rate,spore production yield,sensitivity,counteraction,and genetic stability.The results showed that compared with parent strain T32,resistance of mutant T32-5-10m was raised by 20 times;growth rate and spore production of T32-5-10m was higher than that of T32.The medical resistance of T32-5-10m was steady after 10 successive transfer cultures,and its inhibition rate against 3 soil diseases pathogens was 87.56%～89.11%.
出处
《湖北农业科学》
北大核心
2012年第1期44-47,共4页
Hubei Agricultural Sciences
基金
宁夏回族自治区自然科学基金项目(NZ0980)
关键词
紫外线诱变
拮抗木霉菌株T32
百菌清
抗药性
ultraviolet mutation
antagonistic Trichoderma T32
chlorothalonil
resistance
