银杏幼胚培养的多因子正交试验研究

Studies on Culture of Young Embryo in Ginkgo biloba L. by Orthogonal Design

以MS为基本培养基,选用银杏幼胚为试验材料,采用L9(34)正交设计分析比较2,4-D(A因素)、6-BA(B因素)、酸水解酪蛋白(CH,C因素)3个因素及其组合对银杏幼胚愈伤组织诱导率、褐化率及生长量的影响。结果表明:2,4-D 1.0mg/L+6-BA 1.0mg/L+CH 500mg/L组合银杏的幼胚愈伤诱导率最高,为100%,同时该组合处理愈伤组织生长量也最大,增长倍数达8.11倍;而2,4-D 0.5mg/L+6-BA 0.5mg/L组合褐化率最高,为60.3%。经直观分析、方差分析和主因素效应的多重比较后认为,3个因素中,2,4-D对银杏幼胚愈伤诱导率及褐化率的影响最大,极差分别为74.196和63.702;CH对银杏幼胚愈伤生长量影响最大,极差为2.148。银杏愈伤组织诱导及生长的培养基最优组合为MS+2,4-D 1.0mg/L+6-BA 0.5mg/L+CH 500mg/L。

Using MS as the basic medium and using the embryo of Ginkgo as the material,the research aimed to study the effects of 2,4-D（A）,6-BA（B） and CH（C） in different combinations and different concentrations on callus induction and growth by using orthogonal design L9（34）.The results showed that 2,4-D 1.0 mg/L＋6-BA 1.0 mg/L＋CH 500 mg/L combination had the highest rate of callus induction（100%） and the highest growth rate（8.11 times）.2,4-D 0.5mg/L＋6-BA 0.5 mg/L combination had the highest rate of browning（60.3%）.By intuitive analysis,variance analysis and multiple comparison,results suggest that 2,4-D was the most influential factors in the three tested factors,which have the average range of 74.196 in callus induction and 63.702 in browning.CH had greatest impact on the amount of callus growth,with the average range of 2.148.Therefore,the optimum media for Ginkgo callus induction and growth were MS＋1.0 mg/L 2,4-D＋0.5 mg/L 6-BA＋500 mg/L CH.