摘要
对辣椒杂交种陇椒3号和陇椒5号在不同培养基、不同激素水平下不定芽分化情况进行了研究,结果表明,B5培养基优于MS培养基,更能促进外植体的分化及再生;子叶的分化能力高于下胚轴,是辣椒离体组培的优良外植体;当B5培养基中激素配比组合为在6-BA 5.0 mg/L+NAA 1.0 mg/L时,有利于陇椒3号和陇椒5号的分化再生,最早形成丛生芽。
The two pepper varieties（Capsicum annuum）,Longjiao 3 and Longjiao 5,were used to investigate the effects of such factors as different hormone combinations and culture medium on adventitions bud differentiation and elongation of pepper explants.The results showed that B5 culture medium was better than MS culture medium on adventitions bud differentiation and elongation of pepper explants,and the cotyledons had stronger regeneration capaciation rate.The hormone combin was 6-5.0 mg/L BA＋1.0 mg/L NAA,which was suitable for the regenerative buds.
出处
《甘肃农业科技》
2012年第1期11-14,共4页
Gansu Agricultural Science and Technology
基金
甘肃省自然科学基金资助项目"利用花药培养法诱导辣椒单倍体植株以及DH群体与再生体系的建立"(1010RJZA175)部分内容
兰州市科技项目"耐低温寡照辣椒新品种的选育及示范"(2011-1-152)部分内容
