三氯乙烯染毒对豚鼠肝功能与凋亡基因表达的影响

Effects of trichloroethylene on liver function and apoptosis gene expression in guinea pigs

目的探讨使用三氯乙烯(TCE)染毒对豚鼠肝功能和肝细胞凋亡基因(BAX、BAD、Bc1-2)表达的影响。方法将24只豚鼠随机分为3组,采用豚鼠最大值法(GPMT),设立TCE实验组、阴性对照组、阳性对照组,用皮内注射的方式分别注射TCE、橄榄油、2,4-二硝基氯苯(DNCB),实验结束后观察动物皮肤改变,应用自动生化分析仪检测动物肝功能指标,用荧光定量PCR检测肝细胞凋亡基因表达水平。结果 TCE实验组和阳性对照组动物出现明显皮肤损害。TCE实验组动物血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、乳酸脱氢酶(LDH)活力明显高于阴性对照组(P<0.05或P<0.01)。肝细胞BAX、BAD的mRNA表达水平比阴性对照组显著升高,Bc1-2表达水平下降(P<0.05或P<0.01)。结论三氯乙烯可诱导豚鼠产生明显的皮肤变态反应,引起实验动物肝功能指标改变和肝细胞凋亡基因表达水平明显改变。

[Objective]To study the effects of trichloroethylene（TCE） on liver function and mRNA expression of apoptosis gene BAX,BAD,Bc1-2 in guinea pigs.[Methods]Guinea pig maximization test（GPMT） was applied,24 guinea pigs were randomly divided into 3 groups,that was the negative control group,positive control group and TCE group.Olive oil,2,4-dinitrochiorobenzene（DNCB）,trichloroethylene（TCE） were administrated to the animals with intradermal injection.The changes in animal skin were observed at the end of experiment;the liver function tests were conducted with automatic biochemical analyzer,fluorescence quantitative PCR was used to detect apoptosis gene expression.[Results]Obvious skin damage appeared in the positive control group and TCE group animals,the activities of ALT,AST and LDH increased significantly in the groups of positive control and TCE treatment when compared with the negative control（P0.05 or P0.01）.BAX and BAD mRNA expression increased significantly in TCE group and positive control compared with the negative control group,but Bc1-2 expression decreased in TCE group and positive control（P0.05 or P0.01）.[Conclusion]TCE can induce obvious skin allergy on guinea pigs,and it also affects liver function and apoptosis gene expression obviously.