摘要
在中国油菜是一种可提供高质量油和蛋白的油料作物。双向电泳技术是一种有效的研究蛋白表达方法。综述了该技术的改进及其在油菜研究中的蛋白质提取、营养元素抑制影响、环境抑制影响、生理学研究、及抗菌核病等方面的应用研究,发现该方法可为油菜基因组学研究提供了大量很有价值的信息。随着双向电泳技术的发展,其将在未来的油菜蛋白组学研究中发挥更大的作用。
As rapeseed is a major oil crop with high quality vegetable oil and feed protein in China and the two-dimensional gel electrophoresis （2-DE） is an efficient technique for investigating protein expression, this paper reviews the innovations in 2-DE and its application in the studies of rapeseed. We conclude that 2-DE has provided much valuable information on rapeseed, and it will do more for rapeseed proteomic research in the near future with the technology innovations.
基金
Supported by the Talents Foundation of Hunan Agricultural University(11yj12)
the Foundation of Pre-State Key Laboratory for Germplasm Innovation and Resource Utilization of Crops(11KFXM03)
the National Natural Science Foundation of China(31000722)~~
关键词
油菜
双向电泳
蛋白组学
Rapeseed
Two dimensional gel
Proteomic
