多发性骨髓瘤的免疫表型特征及其意义

Immunophenotype characteristics in multiple myeloma cells and its significance

目的探讨多发性骨髓瘤(MM)的细胞免疫表型特征及意义。方法应用多参数流式细胞术(MPFCM)测定多发性骨髓瘤30例和反应性免疫球蛋白增高症患者15例(对照组)骨髓细胞的免疫表型。结果 MM组CD138和CD38阳性率均为100%,CD56阳性率86.67%,CD117阳性率33.33%,CD19阳性率仅3.33%;对照组CD38、CD138阳性率均为100%,CD19阳性率93.33%,CD56阳性率6.67%,CD117不表达。MM组浆细胞比例及CD138、CD56、CD117表达率均高于对照组(P<0.01),CD19表达率低于对照组(P<0.01)。结论应用MP-FCM同时检测细胞表面CD38、CD138、CD19、CD56、CD117等抗原表达情况,可方便区分骨髓瘤细胞与良性浆细胞,是多发性骨髓瘤的重要诊断依据。

Objective To investigate the immunophenotype characteristics in multiple myeloma(MM)cells and its significance.Methods Four color multiparameter flow cytometry(MP-FCM)was used to test immunophenotype of 30 MM patients and 15 patients with reactive gammopathy.Results The antigen positive expression rale in myeloma cells was as follows: CD138:100%,CD38;100%,CD56:86.67%,GDI 17:33.33%,CD19:3.33%.The results of patients with reactive gammopathy show that CD38 and CD138 were 100%positive,and CD19 was 93.33%positive,CD56 was 6.67%positive, while GD117 was negative.The expression of GD138,GD56 and GD117 in MM group were higher than the control group (P<0.01),expression of CD19 was lower than the control group(P<0.01)Conclusion MP-FCM is an important diagnostic method for MM and detect the GD38,CD138,CD19,CD56 and GD117 can he helpful for distinguishing myeloma cells from plasma cells.