HPLC-ELSD同时测定白英中延龄草苷和去半乳糖替告皂苷的含量

Simultaneous determination of trillin and desgalactotigonin contents in Solanum lyratum by HPLC-ELSD

目的:建立同时测定白英中延龄草苷(trillin)和去半乳糖替告皂苷(desgalactotigonin)含量的HPLC-ELSD法。方法:采用Diamonsil-C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-10 mmol.L-1醋酸铵溶液52∶48,柱温25℃,流速0.6mL.min-1,进样量20μL,检测器漂移管温度为95℃,氮气流速2.3 L.min-1。结果:延龄草苷和去半乳糖替告皂苷分别在20～200 mg.L-1(r=0.999 8)和10～100 mg.L-1(r=0.999 7)内线性关系良好。延龄草苷的平均回收率为99.4%,RSD为0.90%;去半乳糖替告皂苷的平均回收率为100.3%,RSD 1.1%。结论:该方法准确,重复性良好,适用于白英药材的质量控制。

Objective ： To establish a HPLC-ELSD method for simultaneous determination of tfillin and desgalactotigonin contents in Solanum lyratum. Method： A Diamonsil C18 column （4. 6 mm×250mm, 5μm） was adopted, with the mobile phase consisting of acetonitrile-10 mmol· L-1 ammonium acetate （52：48）. The temperature was 25℃, the flow rate was set at 0. 6 mL·min-1 , and the sample size is 20 μL. The temperature of drift tubes and gas flow rate of the detector were set at 95 ℃ and 2. 3 L· min -1, respectively. Result： With in the linear ranges of 20-200 mg· L-1 and 10-100 mg· L-1 , trillin and desgalactotigonin show a good linear relationship. The average recovery was 99.4% （RSD 0. 90% ） for trillin and 100. 3% （RSD 1.1% ） for desgalactotigonin. Conclusion ： The method is so accurate and easily reproducible that it is suitable for the quality control of S. lyratum medicinal materials.