丹酚酸B对大鼠离体心脏缺血再灌注损伤的保护作用

Protective effect of salvianolic acid B on isolated heart ischemia/reperfusion injury in rats

目的:研究丹酚酸B对大鼠离体心脏缺血再灌注损伤的作用及其作用机制。方法:SD大鼠48只,随机分为6组,分别是空白对照组、阳性给药组(维拉帕米150μg.L-1)、丹酚酸B高、中、低剂量组(10,5,2.5 mg.L-1),每组8只。采用Langendorff法灌流离体大鼠心脏,停灌25 min后再灌30 min,造成心肌缺血再灌注损伤模型。于大鼠左心房插入水囊导管,记录丹酚酸B对血流动力学指标的影响,测定不同时间点冠脉流出液中天门冬氨酸氨基转移酶(AST),乳酸脱氢酶(LDH)的活性及心肌组织中超氧化物歧化酶(SOD)活性,丙二醛(MDA)的含量。结果:丹酚酸B不同剂量组(10,5,2.5 mg.L-1),可显著改善缺血再灌注所致大鼠的心功能损伤,减少AST,LDH释放和MDA的产生,增加SOD的活性。结论:丹酚酸B对心肌缺血再灌注损伤具有保护作用,其机制可能与改善心肌舒缩功能,清除氧自由基,减少脂质过氧化反应有关。

Objective： To study the protective effect and mechanism of salvianolic acid B on isolated heart ischemia/repeffu- sion injury in rats. Method： Forty-eight SD rats were divided into 6 groups randomly （n = 8 ） ： the control group, the positive adminis- tration group （ verapamil 150 μg · L -1 ） , and high, middle and low-dose salvianolic acid B groups （ 10,5,2. 5 mg· L-1 ）. The myo- cardial ischemia/reperfusion injury model was established using the Langendorff method, re-perusing isolated working hearts for 30 min after ischemia for 25 rain. A water-bag catheter was inserted in rat left atrium for recording the effect of salvianolic acid B on hemody- namics indexes-AST, LDH, SOD and MDA. Result： Various group with different doses showed that salvianolie acid B decreased AST, release of LDH and formation of MDA and increased SOD activity. Conclusion： Salvianolic acid B showed a protective effect on myo- cardial ischemia/reperfusion injury. Its mechanism may be related with improvement of cardiac contractility, cleaning of oxygen free radicals and reduction of lipid peroxidation.