母乳及婴儿尿液人巨细胞病毒DNA检测在婴儿人巨细胞病毒感染中的应用

Application of the detection of HCMV DNA in urine and breast-milk in infant HCMV infection

目的:定量检测疑似人巨细胞病毒(HCMV)感染婴儿尿液及对应母亲乳汁中HCMV DNA,评估两者在诊断HCMV感染中的价值,并比较两者浓度之间的关系。方法:选取51例疑似HCMV感染的婴儿,收集其新鲜尿液及对应的母亲乳汁,分别用荧光定量聚合酶链式反应法(FQ-PCR)检测HCMV DNA。结果:51例疑似HCMV感染婴儿尿液中,有49例检测出HCMV DNA,阳性率为96.0%(49/51)。51份婴儿母亲乳汁中,有37份检测出HCMV DNA阳性,阳性率为72.5%(37/51)。对37例尿液与乳汁HCMV DNA均为阳性的患儿,分别对尿液与乳汁HCMV DNA浓度取对数,将两者进行相关性分析,结果显示两者之间存在一定的相关性,相关系数为0.332(P<0.05)。结论:婴儿尿液中HCMV DNA检测对于婴儿HCMV的感染具有重要的价值,同时对其母乳HCMV DNA检测,可以发现婴儿尿液和母乳HCMV DNA具有一定的相关性。

Objective To evaluate the value of detecting HCMV DNA in urine and breast-milk in the diagnosis of HCMV infection in infant, and investigate the relationship between their concentrations. Methods The urine and breast-milk samples were collected from 51 infants who displayed clinical symptoms of HCMV infection and their mothers. The HCMV DNA was examined by fluorescence quantitative polymerase chain reaction （FQ-PCR）. Results Of the 51 suspected eases, 49 had detectable HCMV DNA in their urine samples （96.0%）, whereas the positive rate of the relevant breast-milk was 72.5% （37/51）. The correlation analysis between the logarithm HCMV DNA （log HCMV DNA） of urine and breast milk were performed in 37 double-positive subjects with the correlation coefficient of 0.332 （P 〈 0.05）. Conclusions The detection of HCMV DNA in urine is beneficial to the diagnosis of HCMV infection of infants, and the content of HCMV DNA in urine is correlated with that in breast-milk.