食品中大肠杆菌O157:H7的几种检测方法的比较

Comparison of methods detecting Escherichia coli O157:H7 in foods

目的评估平板分离培养法、免疫磁珠分离(IMS)法、VIDAS全自动酶标免疫测试系统、BAX全自动病原菌检测系统及环介导等温扩增(LAMP)技术在食品中检验肠出血型大肠埃希菌O157∶H7的特异性、敏感性。方法使用平板分离培养法、免疫磁珠分离法、VIDAS法、BAX法及LAMP法对人工制备的染菌猪肉样本进行检测,并对这几种方法进行比较。结果 BAX法和LAMP法的检出率最高,分别是89.1%和85.9%,免疫磁珠法和VIDAS法检出率次之,分别是75.0%和78.1%,传统分离培养法为43.8%。结论 BAX法和LAMP法具有快速、高效、特异性好、敏感性高的特点,可快速筛选食品中可能存在的肠出血型大肠埃希菌O157∶H7。

Objective To examine the specificity and sensitivity of traditional cultural method,immunomagnetic beads separation method（IMS）,VIDAS,BAX and Loop-mediated isothermal amplification（LAMP） method for the detection of O157∶H7 in foods.Methods Traditional cultural method,IMS,VIDAS,BAX and LAMP method were used and compared in detecting O157∶H7 in pork samples.Results The detection rate of BAX,LAMP,IMS and VIDAS method was 89.1%,85.9%,75.0% and 78.1% respectively,while that of traditional cultural method was only 43.8%.Conclusion BAX and LAMP method were proved to be rapid,highly effective,specific and sensitive for the detection of O157∶H7 in foods.