摘要
对一株耐As菌株AS-01耐As特性以及耐As机制进行了初步的探索和研究,发现菌株AS-01对As(Ⅲ)的耐受质量浓度最低能达到1 200 mg/L,对As(V)的耐受质量浓度能达到1 600 mg/L以上;菌株As-01对含As废水中含有的其他重金属离子如Cu^(2+)、Zn^(2+)、Ph^(2+)、Cd^(2+)也有不同的耐受能力。通过扫描电镜(SEM)观察发现菌株在500 mg/L的As(Ⅲ)培养液中培养后,茵体细胞间有絮状颗粒物镶嵌;红外光谱分析发现-OH以及酰胺基团是细菌细胞壁上参与结合As(Ⅲ)的官能团。通过质粒消除实验证明该菌株的耐As性质与耐As质粒的存在有关。
The arsenic resistance of a strain AS-01 showed that the minimum tolerant concentration of As （Ⅲ） and As（Ⅴ） for the strain could reached 1 200 mg/L and 1 600 mg/L.The strain AS-01 had various abilities to tolerant other heavy metals,such as Cu^（2＋）,Zn^（2＋）,Pb^（2＋）,and Cd^（2＋） contained in the arsenic contaminated wastewater.The scanning electron microscope（SEM） of AS-01 showed that there were flocculent particles formed on the extracellular surface of the bacterial cultured in the arsenite containing medium.Infrared spectroscopy analysis showed that—OH and amide groups were functional groups in the accumulation of As（Ⅲ）.The strain harbored a plasmid putatively for bearing essential arsenite resistant genes demonstrated by a plasmid elimination experiment.
出处
《南京工业大学学报（自然科学版）》
CAS
北大核心
2012年第1期61-65,共5页
Journal of Nanjing Tech University(Natural Science Edition)
基金
国家自然科学基金资助项目(50678081)
关键词
AS
耐受性
质粒
重金属
arsenic
resistance
plasmid
heavy metal
