不同喂养方法对烫伤大鼠免疫功能康复影响

Effects of different feeding on rehabilitation of immune dysfunction in rats with severe burn

目的观察3种不同喂养方法对烫伤大鼠免疫功能康复影响,探讨促进烧伤机体免疫功能康复方法。方法健康Wistar大鼠100只,随机分成药膳组、肉汤组、常规组(n=30)和对照组(n=10)。药膳组、肉汤组、常规组于伤后3、71、4 d各取10只,正常对照组假伤后立即处死取材,检测T淋巴细胞亚群和自然杀伤细胞(NK)细胞活性、血浆IgAI、gGI、gM、补体C3、C4含量、肠道sIgA含量。结果烧伤药膳组CD3+、CD4+、NK细胞活性分别为(61.87±6.75)%(、33.14±4.91%)(、9.68±1.31)%,IgAI、gGI、gM、C3、C4及肠道sIgA含量分别为(50.24±8.11)(、197.8±23.2)(、161.9±20.6)(、196.0±24.3)(、73.4±9.4)mg/L和(34.6±8.2)μg/mL,均低于对照组,CD 8+([34.33±2.87)%]高于对照组(P<0.05或P<0.01);与肉汤组、常规组比较,药膳组各免疫指标均恢复较快(P<0.05或P<0.01)。结论药膳喂养可以改善烫伤大鼠T淋巴细胞亚群分布,提高NK细胞活性和肠黏膜细胞sIgA水平,促进机体免疫功能康复。

Objective To observe the effect of different feeding on the rehabilitation of immune dysfunction in severely burned rats and to investigate the method of improving the rehabilitation of immune function of severe burn.Methods Totally 100 healthy Wistar rats were randomly divided into group A（n=30,burned and fed with medicinal feeding,group B（n=30,burned and fed with bouillon）,group C（n=30,bured and fed normally）,and group D（n=10,normal control）.The rats in group D were sacrificeded after scalded at 37 ℃.At 3,7,14 day post the burn and 10 rats in group A,B and C were also sacrificed under the condition of asepsis to detect the amount of T lymphocyte subsets,the activity of natuual killer（NK） cells,the levels of IgA,IgG,IgM,C3,C4,and the concentration of secretory immunoglobulin A（sIgA）.Results Compared with group A,B,and C,the amount of the CD＋3,CD＋4,CD＋8,the ratio of CD＋4/CD＋8,the levels of IgA,IgG,IgM,C3,C4,and the activity of NK cells of group D were significantly higher at 3,7,14 day post the burn（（P〈0.05 or P〈0.01）.Compared with group B and C,all the indexes of group A recovered fast（P〈0.05 or P〈0.01）.Conclusion The Medicinal feeding can play an important role in improving the distribution of T lymphocyte,enhancing the activity of NK cells,promoting the secretion ability of of sIgA in intestines mucous membrane,and the levels of IgM,IgG,C3,C4 in rats with severe burn.