间充质干细胞对辐射诱发胸腺瘤β-catenin和c-myc基因影响

Effects of bone marrow mesenchymal stem cell transplantation on expressions of β-catenin and c-myc mRNA of thymoma induced by ionizing radiation in mice

目的探讨骨髓间充质干细胞(MSCs)对辐射诱发胸腺瘤过程中β-catenin和c-myc mRNA表达影响,为阐明MSCs在肿瘤发生发展中作用提供理论依据。方法全骨髓贴壁培养C57BL/6小鼠MSCs,X射线照射C57BL/6小鼠建立胸腺淋巴瘤模型,将小鼠随机分成对照组、辐射组及MSCs组,每组6只;逆转录-聚合酶链反应技术检测各组小鼠胸腺β-catenin和c-myc mRNA表达。结果辐射组小鼠胸腺瘤β-catenin mRNA表达量(0.92±0.23)明显高于对照组(0.64±0.21)(P<0.05),MSCs组β-catenin mRNA表达量(0.77±0.24)下降;与对照组(1.48±0.34)比较,辐射组c-myc mRNA表达量(1.61±0.37)上调,MSCs组c-myc mRNA表达量(1.18±0.34)明显低于辐射组(P<0.05)。结论 MSCs可通过抑制β-catenin和c-myc基因异常表达,抑制肿瘤的增殖。

Objective To study the effects of bone marrow mesenchymal stem cells（MSCs） transplantation on the expressions of β-catenin and c-myc mRNA of thymoma induced by ionizing radiation in mice and to provide theoretical basis for exploring the effect of MSCs in tumor genesis and development.Methods MSCs from C57BL/6 mice were cultured through whole bone marrow adherence in vitro.The model of thymoma was induced by whole-body X-irradiation.The mice were divided into three groups：the normal,irradiation and treatment group with MSCs after irradiation.The expressions of β-catenin and c-myc mRNA were detected by reverse transcription-PCR（RT-PCR） 6 months after the irradiation.Results The expression of β-catenin mRNA in the irradiation group was significantly higher than that of in the nomal control（P〈0.05）,and the expression in the irradiation ＋ MSCs group was lower than that in the irradiation group.The expression of c-myc mRNA in the irradiation group was higher than that of in the nomal control,and the expression in the irradiation＋MSCs group was significantly lower than that in the irradiation group（P〈0.05）.Conclusion MSCs could inhibit tumor proliferation through inhibiting the abnormal expressions of β-catenin and c-myc mRNA.