丹酚酸B对MC3T3-E1细胞Dkk1 mRNA表达的影响

Effect of salvianolic acid B on mRNA expression of Dkk1 in MC3T3-E1 cells

目的:观察丹酚酸B对MC3T3-E1细胞Dkk1mRNA表达的影响。方法:MC3T3-E1细胞分组:正常对照组、成骨诱导组、地塞米松1×10-6 mol·L-1组、地塞米松1×10-6 mol·L-1+丹酚酸B1×10-7 mol·L-1组、地塞米松1×10-6 mol·L-1+Dkk1抗体100μg·L-1组、丹酚酸B1×10-7 mol·L-1组、Dkk1抗体100μg·L-1组。加药7 d后用RT-PCR法检测细胞Dkk1mRNA表达水平。结果:与正常组相比,成骨诱导后Dkk1的表达增多,地塞米松组的Dkk1表达明显增多,丹酚酸B组Dkk1的表达减少,Dkk1抗体单用未见Dkk1的表达。与地塞米松组相比,丹酚酸B单用Dkk1的表达比地塞米松组少。丹酚酸B、Dkk1抗体可对抗地塞米松应用后引起的Dkk1表达增多。结论:丹酚酸B可以减少Dkk1的表达和对抗地塞米松使用后引起的Dkk1表达增多,这可能是其促骨形成的机制之一。

OBJECTIVE To investigate the effect of salvianolic acid B（Sal B） on mRNA expression of Dkk1 in MC3T3-E1 cells.METHODS The mRNA expression of Dkk1 was detected by RT-PCR after the cells cultured with the following designed groups for 7 days： control group,osteogenic induction group,dexamethasone（DEX） group（1×10^-6 mol·L^-1,anti-Dkk1 antibody group（100 g·L-1）,Sal B group（1×10^-7 mol·L^-1,Sal B（1×10^-7 mol·L^-1 in combination with DEX（1×10^-6 mol·L^-1 group,anti-Dkk1 antibody（100 μg·L^-1） in combination with DEX（1×10^-6 mol·L^-1 group.RESULTSThe osteogenesis-inducing medium and DEX up-regulated Dkk1 mRNA expression compared with the control group.Sal B down-regulated Dkk1 mRNA expression compared with the control group.The mRNA expression of Dkk1 decreased while the cells culture with Sal B compared with the DEX group.Sal B and anti-Dkk1 antibody had antagonistic effect against DEX-induced Dkk1 mRNA expression.CONCLUSION Salvianolic acid B can induce the osteogenic differentiation by decreasing Dickkopf-1 mRNA expression.