罗红霉素与乙酰螺旋霉素对眼部金黄色葡萄球菌生物膜的影响

Synergism of roxithromycin and acetylspiramycin on Staphylococcus aureus biofilm in ocular region

目的:比较罗红霉素与乙酰螺旋霉素对眼部金黄色葡萄球菌生物膜的影响及与环丙沙星合用的抗菌协同作用。方法:用0.22μm微孔膜构建金黄色葡萄球菌生物膜模型;测定罗红霉素、乙酰螺旋霉素及环丙沙星的最低抑菌浓度(MIC)值;银染金黄色葡萄球菌生物膜,扫描电镜(SEM)观察生物膜形态结构,连续稀释法进行活菌计数。结果:生物膜(BF)可在7 d内稳定形成,1/16MIC、1/4MIC浓度罗红霉素显著减少单用环丙沙星组中的BF活菌数(P<0.05),乙酰螺旋霉素没有明显作用。结论:罗红霉素能显著提高环丙沙星对BF中金黄色葡萄球菌的抗菌活性。

OBJECTIVE To compare the influence of roxithromycin and acetylspiramycin on Staphylococcus aureus biofilm formation in ocular region and the Synergism of antibacterial activities between roxithromycin and ciprofloxacin on staphylococcus aureus.METHODS In vitro Staphylococcus aureus biofilm were cultivated with 0.22 μm aperture microporefilm.The MICs of roxithromycin and acetylspiramycin and ciprofloxacin were measured;Staphylococcus aureus BF was stained with AgNO3 and the morphological structure was observed by scanning electron microscopy（SEM）;The viable bacterial in BF were counted separately by serial dilution.RESULTS The BF of Staphylococcus aureus was formed steadily after a week.The viable bacterial amount showed when Roxithromycin of 1/16MIC and 1/4MIC combined with Ciprofloxacin alone was applied respectively,bactericidal activity of Ciprofloxacin to Staphylococcus aureus within the BF was enhanced significantly（P〈0.05）.Acetylspiramycin showed without effect.CONCLUSION Roxithromycin was considered to enhance significantly the bacterial sensitivity to ciprofloxacin on Staphylococcus aureus in biofilm.