离子交换树脂对钩藤总生物碱的纯化工艺

Purification Technology of Total Alkaloids from Uncaria rhynchophylla by Ion-Exchange Resin

目的:建立阳离子交换树脂纯化钩藤总生物碱的工艺。方法:以钩藤总生物碱为研究指标,考察离子交换树脂分离钩藤总生物碱的工艺条件。结果:选择732型阳离子交换树脂,以1 mL.min-1的流速上样,分别用10 BV水,6 BV 80%乙醇洗脱除杂,最后选用1%氨性80%乙醇洗脱得到钩藤总生物碱,含量达到50%。结论:该方法工艺较简单,总碱含量较高,适合工业化生产。

Objective：To establish purification technology of total alkaloids from Uncaria rhynchophylla with cation-exchange resin.Method：Total alkaloids from U.rhynchophylla was selected as indicator,and investigated separation technology conditions of total alkaloids from U.rhynchophylla by ion-exchange resin.Result：Seven hundred and thirty-two cation-exchange resin was chosen,flow rate of sample was 1 mL·min-1 of flow rate,then eluted with 10 BV water and 6 BV 80% ethanol,finally eluted with 1% aqueous ammonia 80% ethanol,the content of total alkaloids from U.rhynchophylla was up to 50%.Conclusion：This technology was simply,the content of total alkali was high,suitable for industrial production.