白金妇安颗粒中挥发油β-环状精包合物制备工艺

Preparation Technology of β-Cyclodextrin Inclusion Compound for Volatile Oil from Baijin Fuan Particle

目的:优选白金妇安颗粒中挥发油β-环状精包合物制备工艺。方法:以不同时间挥发油的提取量为指标,采用正交试验法对白金妇安颗粒中的当归、川芎、郁金、牡丹皮的挥发油提取工艺进行优选;以倍他环糊精(β-CD)包封挥发油的包封率为指标,采用正交试验法对白金妇安颗粒中的的挥发油包合工艺进行优选。结果:药材当归、川芎、郁金、牡丹皮粉碎成粗粉,加5倍量的水,浸泡2 h,水蒸气蒸馏法提取挥发油5 h。取挥发油5倍量的β-环糊精,加入1倍β-CD量20%乙醇,研磨成糊状,加入挥发油,继续研磨2 h,得糊状物,低温干燥。结论:确定的工艺合理,有效成分提取率高。

Objective：To study on purification technology conditions of total flavonoids from Carthamus tinctorius by macroporous resin.Method： Absorption rate and desorption rate of total flavonoids were taken as indexes,investigated absorption and desorption ability of 6 different types macroporous resin to safflower flavonoids,determined AB-8 was optimum macroporous resin.Purification conditions of AB-8 macroporous resin were optimized.Result：Optimum technology conditions were as follows：liquid concentration was 50 g·L-1（pH 2）,flow rate of sample was 4 BV·h-1;eluted with 3 BV the amount of 30% ethanol by speed of 2 BV·h-1.After purified with AB-8 macroporons resin,purity of total flavonoids was up to 54.04%,purity of hydroxysafflor yellow A was up to 28.76%,separation effect was good.Conclusion： AB-8 resin was fit for separating and purifying of total flavonoids from safflower.