摘要
目的:优选白金妇安颗粒中挥发油β-环状精包合物制备工艺。方法:以不同时间挥发油的提取量为指标,采用正交试验法对白金妇安颗粒中的当归、川芎、郁金、牡丹皮的挥发油提取工艺进行优选;以倍他环糊精(β-CD)包封挥发油的包封率为指标,采用正交试验法对白金妇安颗粒中的的挥发油包合工艺进行优选。结果:药材当归、川芎、郁金、牡丹皮粉碎成粗粉,加5倍量的水,浸泡2 h,水蒸气蒸馏法提取挥发油5 h。取挥发油5倍量的β-环糊精,加入1倍β-CD量20%乙醇,研磨成糊状,加入挥发油,继续研磨2 h,得糊状物,低温干燥。结论:确定的工艺合理,有效成分提取率高。
Objective:To study on purification technology conditions of total flavonoids from Carthamus tinctorius by macroporous resin.Method: Absorption rate and desorption rate of total flavonoids were taken as indexes,investigated absorption and desorption ability of 6 different types macroporous resin to safflower flavonoids,determined AB-8 was optimum macroporous resin.Purification conditions of AB-8 macroporous resin were optimized.Result:Optimum technology conditions were as follows:liquid concentration was 50 g·L-1(pH 2),flow rate of sample was 4 BV·h-1;eluted with 3 BV the amount of 30% ethanol by speed of 2 BV·h-1.After purified with AB-8 macroporons resin,purity of total flavonoids was up to 54.04%,purity of hydroxysafflor yellow A was up to 28.76%,separation effect was good.Conclusion: AB-8 resin was fit for separating and purifying of total flavonoids from safflower.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第3期36-38,共3页
Chinese Journal of Experimental Traditional Medical Formulae