女贞子多糖的提取工艺及单糖组成

Optimum Extracting Method and Monosaccharide Composition of Polysaccharide from Ligustrum lucidum

目的:优选女贞子多糖的最佳提取工艺并测定其单糖组成及摩尔比。方法:采用正交试验法,以多糖提取率为评价指标,对女贞子多糖的提取因素进行研究,用苯酚-硫酸比色法对女贞子多糖进行含量测定。多糖经硫酸水解后,水解产物利用高效液相色谱(HPLC)分析其单糖组成及摩尔比。结果:女贞子多糖的最佳提取工艺为10倍量水,回流提取3次,3 h/次;该多糖由鼠李糖、阿拉伯糖、甘露糖和葡萄糖4种单糖组成,其摩尔比为3.71∶3.21∶1.00∶16.06。结论:该方法简便、准确,可用于女贞子多糖的单糖组成测定及质量控制。

Objective：To optimize the extraction process of polysaccharide from Ligustrum lucidum Ait.（PLLA） and analyze monosaccharide compositionof PLLA.Method：With the extraction efficiency of polysaccharide as the index,an orthogonal design test was adopted to optimize the extraction process.The phenol-sulphuric acid colorimetric method was used to determine the content of PLLA.The PLLA was hydrolyzed by sulfuric acid,the hydrolyzate was assayed by HPLC.Result：The best extraction process of PLLA was added 10 times amount of water for 3 times and 3 hours for each time at the temperature of 100 ℃.PLLA composed of rhamnose,arabinose,mannose,glucose and their molratio is 3.71∶3.21∶1.00∶16.06.Conclusion： The method is simple,quick and accurate.This method could be applied for the analysis of monosaccharide composition and quality control in PLLA.