扶正解毒颗粒对染镍大鼠肾组织核因子-κB活化的影响

Effects of Fuzheng Jiedu Granula on the Activation of NF-κB in Rats Exposed to Nickel

目的:研究中药扶正解毒颗粒(FJG)对硫酸镍(NiSO4)染毒大鼠肾组织核因子-κB(NF-κB)活化的影响。方法:50只Wistar大鼠采用NiSO4 2.5 mg.kg-1 ip(连续7 d,此后间日1次)制备肾损伤模型,随机分为NiSO4组(模型组)、FJG高、中、低剂量组(分别为20,10,5 g.kg-1.d-1),二硫基丁二酸(DMSA),50 mg.kg-1.d-1)组。另设NS组(空白对照)及FJG对照组(FJG 10 g.kg-1.d-1)。各组大鼠ig 1次/d,共4周。测定各组大鼠血尿素氮(BUN)、肌酐(SCr)及24 h尿蛋白定量(24 h-UP),免疫组化SP法观察肾组织NF-κB活化。结果:NiSO4组大鼠出现肾功能损伤,NF-κB活化:肾小球(34.62±9.11)%,肾小管(62.45±14.78)%;FJG大剂量组NF-κB活化:肾小球(2.08±0.64)%,肾小管(11.48±3.39)%,明显低于NiSO4组(P<0.01),肾功能损伤亦明显减轻(P<0.05,P<0.01)。结论:FJG可能通过抑制肾组织NF-κB活性的异常升高而发挥拮抗镍性肾损伤的作用。

Objective：To study the effects of Fuzheng Jiedu Granula（FJG） on the activation of nuclear factor-kappa-B（NF-κB） in rats exposed to nickel（nickel sulfate,NiSO4）.Method： Rat nephrotoxicity model was established by intraperitoneal injection of NiSO4（2.5 mg·kg-1·d-1）.They were randomized into 5 groups,model group,FJG high dose group（20 g·kg-1·d-1）,FJG middle dose group（10 g·kg-1·d-1）,FJG,low dose group（5 g·kg-1·d-1）,meso-2,3-dimercaptosuccinic acid（DMSA） group（0.05 g·kg-1·d-1）.Twenty Wistar rats were randomly divided into NS group and FJG control group（10 g·kg-1·d-1）.Rats in each group were treated by intragastric administration once every day for four weeks.The levels of blood ureanitrogen（BUN）,Creatinine（Scr） and 24 hours-proteinuria（24 h-UP） were measured.The activation of NF-κB was measured with immunohistochemistry methods.Result： In FJG groups,the levels of BUN,Scr,24h-UP and the activation of NF-κB were all decreased（P0.05,P0.01）,compared with that of NiSO4 group.Conclusion： Inhibiting the activation of NF-κB,FJG could protect renal from injury induced by nickel.