小柴胡汤对体外新生大鼠柯萨奇病毒B_3心肌炎模型的酶学与细胞病变的影响

Influence of Xiaocaihu Decoction on Myocardial Enzymes and Cytopathic Effect in Newborn Rat Coxsackievirus B_3 Myocarditis in vitro

目的 探讨中药小柴胡汤对病毒性心肌炎的心肌酶学和细胞病变的影响及对病毒性心肌炎的治疗作用。 方法 体外培养新生大鼠心肌细胞 ,并感染柯萨奇病毒 B3(Coxsackievirus B3,CVB3) ,以此作为体外病毒性心肌炎模型 ,观测心肌细胞感染 CVB32 4、48、72、96 h后乳酸脱氢酶(L DH )、β-羟丁酸脱氢酶 (HBDH )和细胞病变 ,并与小柴胡汤治疗组相比较。 结果 小柴胡汤对CVB3感染心肌细胞 2 4、48、72、96 h的心肌酶升高和细胞病变有显著的抑制作用。 结论 小柴胡汤具有抗柯萨奇病毒感染和保护心肌细胞作用。

Objective To explore the influence of Xiaocaihu Decoction on myocardial enzymes and myocardial cytopathic effect (CPE) in viral myocarditis and its therapeutic effect. Methods Using infected newborn rat myocardial cell cultured with Coxsackievirus B 3 (CVB 3) as a viral myocarditis model in vitro, we observed lactic acid dehydrogenase (LDH), hydroxybutyrate dehydrogenase (HBDH) and myocardial CPE of myocardial cells at 24,48,72,96 hours after infection of CVB 3 and compared it with that of Xiaocaihu Decoction treatment group. Results Xiaocaihu Decoction had significantly inhibitory effect on myocardial enzymes elevation and myocardial CPE at 24,48,72,96 hours after infection of CVB 3. Conclusion Xiaocaihu Decoction has anti Coxsackievirus infection and protecting myocardial cells effect.