芪苈强心胶囊对心气虚型慢性心力衰竭大鼠心肌腺苷酸含量的影响

Effect of Qili Qiangxin Capsule on Adenosine Content in Chronic Heart Failure Rats with Insufficiency of Heart-Qi

目的:观察芪苈强心胶囊对心气虚型慢性心衰大鼠缺血心肌中腺苷酸[三磷酸腺苷(ATP)、二磷酸腺苷(ADP)、一磷酸腺苷(AMP)]含量的影响。方法:采用结扎大鼠冠状动脉左前降支,心肌梗死8周后形成心气虚型慢性心力衰竭模型,随机分为假手术组、模型组、芪苈强心胶囊高、中、低(1.0,0.5,0.25 g.kg-1)剂量组、缬沙坦20 mg.kg-1组。连续给药4周后,利用高效液相色谱法,检测大鼠心脏缺血心肌中ATP,ADP,AMP含量,计算能荷。结果:模型组大鼠心肌组织中ATP,ADP,AMP含量分别由(8.96±2.68),(63.66±14.34),(201.6±66.80)mg.g-1下降至(5.75±1.19),(48.4±15.62),(133.4±54.53)mg.g-1,与假手术组比较具有显著差异(P<0.01),芪苈强心胶囊高剂量组能明显增加缺血心肌中ATP含量,提高能荷,与模型组比较有明显差异(P<0.05)。结论:芪苈强心胶囊可增加缺血心肌组织中腺苷酸含量,从而改善心肌梗死后心衰大鼠心肌能量代谢。

Objective： To observe the effect of Qili Qiangxin capsule on the content of adenosine triphosphate（ATP）,adenosine diphosphate（ADP）,adenosine monophosphate（AMP） in myocardium of heart failure rats.Method： The anterior descending coronary artery was ligated to result in myocardial infarction and establish congestive heart failure（CHF） model with insufficiency of Heart-Qi.After 8 weeks symptoms of insufficiency of Heart-Qi appeared.Rats were randomly divided into sham operation group,model group,Qili Qiangxin capsule groups with dose of 0.25,0.5,1.0 g·kg-1 and valsartan group.After 4 weeks of continuous administration,high performance liquid chromatography（HPLC） was used to detect the content of ATP,ADP,AMP in ischemia myocardium,and the energy charge was calculated.Result：Compared with sham group,the content of ATP,ADP,AMP in myocardium was obviously decreased in model group（P0.01）.Compared with model group,the content of ATP in high dose group of Qili Qiangxin capsule was obviously increased（P0.05）.The high dose group of Qili Qiangxin capsule could increase the energy charge（P0.05,compared with the model group）.Conclusion： Qili Qiangxin capsule can effectively inhibit the obstacle of energy metabolism and protect the cardiac function by up-regulation the contents of ATP.