赤芍煎剂防治急性胰腺炎模型大鼠的作用观察

Effect of red peony root decoction on rats with severe acute pancreatitis

目的观察赤芍煎剂对急性胰腺炎（acute pancreatitis，AP）模型大鼠的治疗效果及对核因子kappaB（NF-κB）、肿瘤坏死因子．α（TNF-α）、白细胞介素-6（IL-6）表达的影响。方法SD大鼠40只采用完全随机分组方法分为5组：正常对照组（A组）、AP模型24h组（B组）、AP模型72h组（C组）、24h赤芍煎剂组（D组）、72h赤芍煎剂组（E组），每组8只。采用L-精氨酸腹腔注射诱发急性胰腺炎，造模后分别给予生理盐水（B、C组）、赤芍煎剂（D、E组）灌胃，于造模后24、72h点处死各组大鼠。采血检测血清淀粉酶、TNF-α、IL-6；取胰腺组织分为2份：HE染色行光镜检查、Western Blot法检测NF-κB p65蛋白表达。结果与A组比较，B、D组大鼠血清淀粉酶均升高[分别为（3569±516）U／L、（2502±385）U／L，P〈0．05]。但D组的血清淀粉酶水平明显低于B组（P〈0．05）。C、E组血清淀粉酶水平均降低，接近A组[分别为（1279±142）U／L、（1421±203）U／L，P〉0．05]。D组与B组比较、E组与C组比较，病理改变和病理学评分均减轻。血清TNF-α、IL-6的水平：D组为（12．54±2．12）gg／ml、（552．88±81．27）pg／ml，明显低于B组（20．53±4．16）pg／ml、（1255．75±118．82）pg／ml；E组为（11．01±2．02）pg／ml、（582．87±88．86）pg／ml，明显低于C组（23．85±5．59）pg／ml、（1366．33±163．16）pg／ml。Western Blot结果显示，D组的NF-κB p65／β-actin比值（0．07±0．055）低于B组（0．71±0．029），E组（0．15±0．038）低于C组（0．65±0．026）。结论赤芍煎剂对AP模型大鼠的作用机制可能与其抑制NF-κB活化、在整体水平上抑制细胞因子基因表达有关。

Objective To observe the therapeutic effect of red peony root decoction （RPRD） on model rats with acute pancreatitis （AP）, and the effect on the expression of NF-κB, TNF-α and IL-6. Methods Forty SD rats were randomly divided into five groups： normal control group （group A）, AP model groups （group B, 24 h; group C, 72 h） and RPRD treatment groups （group D, 24 h; group E, 72 h）. There were 8 rats in each group. The AP models were induced by intraperitoneally injecting with L-arginine. After the model was established, rats of group B and group C were treated with intra-gastric instillation of NS, and rats in group D and group E with RPRD. At 24 and 72 hours after induction of AP, rats in each group were sacrificed and blood samples were taken for the measurement of serum amylase, TNF-α and IL-6. The expression of NF-κB p65 protein in the pancreas were detected by Western Blot, and pathological status of the pancreas were analyzed. Results The serum amylase level in group B and group D was significantly higher than that in group A （P〈 0.05）, and the level in group D was obviously lower than that in group B （P〈0.05）. The serum amylase level in group C and group E was decreased, and there was no difference between group A （P〉0.05）. The pathological severity and pathological scores of the pancreas in group D and group E were more lighter than those in group B and group C （P〈0.05）. The level of serum TNF-α and IL-6 was significantly decreased in RPRD treatment groups （P〈 0.05）, group D compared with group B, [ （12.54± 2.12） pg/ml, （552.8± 81.27） pg/ml] vs. E （20.53 ±4.16） pg/ml, （1255.75±118.82）pg/ml]; group E compared with group C, [ （11.01±2.02）pg/ml, （582.87＋88.86）pg/ml] vs [ （23.85±5.59）pg/ml, （1366.33± 163.16）pg/ml]. The expression levels of NF-κB p65 protein in the pancreas of group D and group E were significantly lighter than those in group C at both time points （group D compared with group B, （0.07±0.055） vs （0.71±0.029）, P〈0.05; group E compared with group C, （0.15±0.038） vs （0.65±0.026）. Conclusion RPRD may alleviate the pathologic changes in AP model rats, the mechanism may involve its inhibiting the activity of NF-κB and expression of TNF-α and IL-6.