补肾中药对糖皮质激素性骨质疏松模型大鼠的干预作用

Treating effects of nourishing-kidney herbs on rat models with glucocorticoid-induced osteoporosis

目的采用地塞米松肌注法建立糖皮质激素性骨质疏松（glucocorticoid-inducedosteoporosis，GIO）大鼠模型，观察补肾中药的防治作用。方法Wistar大鼠60只，雌雄各半，按体重分层随机法分为正常组、模型组、补肾组。均采用肌注地塞米松的方法造模，2．5mg／kg·次^-1，2次／周，连续9周。测定离体股骨骨密度（BMD）及血清骨代谢指标。结果模型组模型大鼠BMD明显降低（0．109±0．007）g／cm^2，血清骨吸收标记物TRAP含量明显升高（9．96±1．15）μg／ml；补肾组模型大鼠BMD（0．116±0．007）g／cm^2，血清TRAP含量降低（5．76±O．85）μg／ml。结论补肾中药对糖皮质激素诱导的GIO大鼠模型有一定拮抗作用。

Objective To observe the effects of nourishing-kidney herbs on rat model with glucocorticoid-induced osteoporosis. Methods 60 Wistar rats male and female half and half were randomly divided into a normal group, a model group and a nourishing-kidney group. By intramuscular injection of dexamethasone （2.5 mg/kg） twice a week to replicate osteoporosis rat model. All groups were treated for 9 weeks. Detected the BMD of femur in vitro and determined the bone metabolism marker in serum by biochemical process. Results The BMD decreased obviously （0.109± 0.007）g/cm^2 and the content of TRAP in serum increased evidently （9.96± 1.15） μg/ml in the model group. In the nourishing-kidney group, the BMD was up-regulated （0.116 ± 0.007）g/cm^2, and TRAP down-regulated（5.76 ±0.85）μg/mh Conclusion Intramuscular injection of dexamethasone can induce GIO rat model, and nourishing-kidney herbs have the effect of anti-osteoporosis.