棓丙酯脂质体的制备与表征

Preparation and in vitro characterization of Propylgallate liposomes

目的制备棓丙酯脂质体,并对其进行理化性质的表征和释放度的评价。方法采用薄膜分散法制备棓丙酯脂质体,超滤离心法测定脂质体的包封率,正交设计优化处方,并对其包封率、粒径、Zeta电位、形态及体外释放行为进行综合评价。结果正交设计优化最终处方为磷脂浓度5 mg.mL-1、药脂比1∶5、磷脂胆固醇比5∶1、水化介质离子强度20 mmol.mL-1,所得脂质体包封率为89.6%、粒径为181.3 nm、Zeta电位为-21.8 mV、4 h体外释放达到80%。结论制备的棓丙酯脂质体包封率高,粒径小而均一,体外释放完全。

OBJECTIVE To prepare and characterize Propylgallate（PG） liposomes.METHODS PG liposome was prepared by film dispersion method and ultrafiltrate centrifugation method was used to determine encapsulation efficiency.RESULTS The optimized formulation parameters included：the concentration of soybean lecithin（SPC） 5 mg·mL-1,PG-SPC（1：5）,SPC-cholesterol（5：1）,ionic strength 20 mmol·L-1.The particle diameter was 181.3 nm and Zeta potential was-21.8 mV.The cumulative of release amount was up to 80% within 4 h.CONCLUSION PG liposomes are of high encapsulation efficiency with a small and uniform particle size.