中药不同组方对胃癌前病变模型大鼠胃黏膜细胞Caspase-3表达的影响

Expression of Caspase-3 in gastric tissue of rats with precancerous lesions treated by different traditional Chinese herbs

[目的]探讨不同组方中药对慢性萎缩性胃炎伴不典型增生大鼠胃黏膜形态、组织学改变的影响。[方法]采用化学药物刺激的方法复制慢性萎缩性胃炎癌前病变动物模型并分组用药物干预。用酶标仪检测各组大鼠胃组织中Caspase-3表达的变化。[结果]无论是预防组还是治疗组,中药、西药的干预均可以使Caspase-3的表达增加,与模型大鼠比较差异有统计学意义(P<0.05);健脾化瘀方要优于其他药物。[结论]各组中药均可以通过增加胃组织中Caspase-3的表达达到对胃癌前病变的治疗作用,其中健脾化瘀方治疗萎缩性胃炎癌前病变的作用要明显优于健脾益气方、活血化瘀方。

[Objective]To explore the effects of different traditional Chinese herbs on gastritic morphological and histological changes in rats with chronic atrophic gastritis with dysplasia. [Methods]The animal models of chronic atrophic gastritis with precancerous lesions were made by using chemical substances,and then derided into different groups for therapy. The expression of Caspase-3 in gastric tissue was detected by microplate reader for enzyme activity. [Results]Either in prevention or treatment groups, the expressions of Caspase-3 were significantly increased by the intervention of TCM and western medicine, P〈 0.05 when compared with the model group. Jianpi Huayu Decoction group was better than other groups. [Conclusion] TCM with the function of strengthening spleen-qi and smoothing blood stagnation（Jianpi Huayu）, strengthening spleen-qi（Jianpi）, and smoothing blood stagnation（ Huayu） played an important role in the prevention and treatment of the gastric epithelial dysplasia. The mechanism might be contributed to the increase of the Caspase-3 expression in gastric tissue. TCM with the function of Jianpi Huayu were better than the other two treating principles（Jianpi and Huayu） in the treatment of atrophic gastritis with precancerous lesions.