温阳活血方延缓5/6肾切除大鼠肾功能衰竭的实验研究

Experimental Research of "Wenyang Huoxue Prescription" in Retarding Renal Failure in Rats Following 5/6 Nephrectomy

目的:探讨温阳活血方对5/6肾切除大鼠肾功能衰竭的干预作用。方法:选用120只SD雄性大鼠,建立5/6肾切除大鼠模型。随机分为5组:假手术组、模型组、温阳活血方组、科素亚组、温阳活血方+科素亚组。分别在给药后4、8、12周,每组随机选取8只大鼠处死,观察肾组织病理变化,检测肾功能指标。结果:肾脏病理显示,模型组肾小球硬化,肾小管萎缩或消失,肾间质伴有一定程度的纤维化。3个治疗组病理改变有不同程度的好转,温阳活血方组和温阳活血方+科素亚组肾小球硬化程度轻于科素亚组。与假手术组比较,模型组尿素氮(BUN)、血肌酐(Scr)、血清胱抑素C(Cys-C)和24h尿蛋白、尿N-乙酰-β-D氨基葡萄糖苷酶(尿NAG)明显均明显升高(P<0.01,P<0.05);3个治疗组BUN、Scr、Cys-C和24h尿蛋白、尿NAG明显低于模型组(P<0.01)。结论:温阳活血方对慢性肾衰竭大鼠肾功能有一定的保护作用,能降低慢性肾功能衰竭大鼠的Scr、BUN、Cys-C和24h尿蛋白、尿NAG水平。

Objective： To investigate the intervention effects of ＂Wenyang Huoxue Prescription＂（WYHXF） on 5/6 nephrectomized rats.Methods： One hundred and twenty SD rats were used to establish the 5/6 nephrectomized models,and then randomized into 5 groups： sham-operation group,model group,WYHXF group,Losartan group and combination（Losartan combined WYHXF） group.After the treatment,eight rats in each group were sacrificed at 4th,8th and 12th weeks,and the indexes of renal failure were detected.Results： Renal pathology showed glomerulosclerosis,tubular atrophy or disappearance,interstitial fibrosis in model group.Pathological changes in three treatment groups with different degrees of improvement,glomerulosclerosis was milder in WYHXF group and Losartan group than that in combination group.Compared with sham-operation group,the BUN,Scr,Cys-C,24-hour urinary protein and urinary N-acetyl-β-D-glucosaminidase in model group were significantly increased（P〈0.01,P〈0.05）;the BUN,Scr,Cys-C,24-hour urinary protein and urinary N-acetyl-β-D-glucosaminidase in three treatment groups were lower than those in model group（P〈0.01）.Conclusion： ＂Wenyang Huoxue Prescription＂ has protective effects on renal function by reducing the levels of BUN,Scr,Cys-C,24-hour urinary protein and urinary N-acetyl-β-D-glucosaminidase in rats with chronic renal failure.