荧光寿命的锁相检测技术

Phase-Locking Detection Technique of Fluorescence Lifetime

荧光寿命作为荧光信号的本征参量,其精确测量对于荧光检测和分析技术具有非常重要的意义。根据荧光信号的特点和荧光寿命的定义,介绍了荧光寿命锁相检测技术的原理,设计了基于锁相环电路的荧光寿命检测系统,并将该系统用于荧光法溶解氧测量,实验结果表明,荧光寿命的测量值与实际值相对误差小于1.5%,系统响应时间小于60 s,可实现荧光寿命的高精度实时监测。

As the intrinsic parameter of fluorescence signal,the accurate measurement of fluorescence lifetime is very important to fluorescence detecting and analyzing technique.According to the characteristics of fluorescence signal and definition of fluorescence lifetime,principle of phase-locking detection technique of fluorescence lifetime is introduced.Based on phase locked loop circuit,fluorescence lifetime detecting system is designed,which is used to dissolved oxygen detecting with fluorescence method.The experimental results showed that the relative error of measured values and actual values is less than 1.5%,and the response time is less than 60 s.