胃脘舒冲剂中甘草酸的HPLC法定量

Determination of Glycyrrhizin in Weiwanshu Granule by HPLC

测定胃脘舒冲剂中甘草酸的含量。采用高效液相色谱法,以对羟基苯甲酸丁酯为内标物,甲醇∶水∶36% 醋酸(65∶28∶7) 为流动相,Shimpack CLCODS柱(6-0m m ×150m m) ,流速1-0ml/min,检测波长254nm 。结果:此法线性范围18～300μg/ml,r=0-9999(n = 5) ,加样回收率为98-35% ,RSD 为3-39 % (n = 9) ,日内变异1-63 % ,日间变异3-35 % 。

The content of glycyrrhizin in weiwanshu granule was determined by high performance liquid chromatography.Chromatographic condition were as follows:internal standard:n butyl p hydroxybenzoate;mobile phase:methanol∶water∶36% acetic acid (65∶28∶7);column size:Shim pack CLC ODS(6 0mm×150mm);flow rate:1 0ml/min and detection wavelength:254nm.The method was linear within the range of 18～300μg/ml( r=0 9999,n=5) .The average recovery, RSD,RSD of within day and day to day were 98 35%,3 39%( n =9),1 63% and 3 35%,respectively.This method was sensitive and highly reproducible.