抗纤复方影响大鼠肝贮脂细胞生成Ⅰ、Ⅲ型胶原的比较研究

Effect of Kangxian Chinese Prescription on Production of Collagen Type Ⅰ and Ⅲ of Hepatic Fat Storing Cells

用免疫细胞化学染色法观察抗纤复方药物血清对大鼠正常肝传代贮脂细胞和纤维肝原代贮脂细胞Ⅰ、Ⅲ型胶原表达的影响,并对Ⅰ型和Ⅲ型胶原的表达量进行比较分析。结果:传代贮脂细胞Ⅲ型胶原增加显著,而纤维肝贮脂细胞Ⅰ型胶原增加明显,抗纤复方能显著抑制贮脂细胞表达胶原,且对传代贮脂细胞Ⅲ型胶原和纤维肝原代贮脂细胞Ⅰ型胶原抑制作用更明显。

The effects of the drug containing sera from Kangxian Chinese prescription on the expression of collagen type Ⅰand Ⅲ in subcultured fat storing cells from normal liver and primary fat storing cells from fibrous liver of rats were investigated by using immunocytochemical staining. The prescription could significantly inhibit the expression of collagen in fat storing cells.The action was stronger on collagen type Ⅲ in subcultured fat storing cells and collagen type Ⅰ in primary fibrous hepatocytes.