相干拉曼散射显微术

Coherent raman scattering microscopy

相干拉曼散射显微术(Coherent Raman Scattering Microscopy)是一类植根于拉曼散射的光学显微成像方法,主要包含相干反斯托克斯拉曼散射(Coherent Anti-Stokes Raman Scattering,CARS)和受激拉曼散射(Stimulated Raman Scattering,SRS)两种方法.CARS/SRS显微术通过探测目标分子特定的振动来提供成像所需的衬度,通过非线性光学过程大大提高了检测的灵敏度,同时本征地具备三维成像能力.CARS和SRS显微术可以对脂类等不易被标记的物质成像,还可以很好地通过选择振动光谱,对生物体内特定小分子物质如药物等,以及生物大分子如核酸、蛋白质等进行无需标记的成像,因此成为极有潜力的活体(invivo)成像手段.本文主要介绍了CARS和SRS显微术的基本原理、实验操作及其在化学和生命科学中的应用.

Coherent Raman Scattering Microscopy is an optical imaging method rooted in Raman Scattering.It involves Coherent Anti-Stokes Raman Scattering（CARS） and Stimulated Raman Scattering（SRS）.CARS and SRS offer chemical contrast via detecting vibration of chemical bonds.Both CARS and SRS are nonlinear optical processes,offering high sensitivity and 3-dimensional sectioning capability intrinsically.As label-free imaging technologies,CARS and SRS have been employed to observe substance that is hard to label,such as lipids and specific small molecules including metabolites,and large biomolecules including proteins and nucleic acids.These features also ensure CARS and SRS as promising imaging methods for in vivo studies.In this paper,we focus on the principle,methodology,as well as the applications of CARS and SRS microscopies in chemistry and life sciences.