三氧化二砷大椎穴注射对哮喘大鼠细胞因子的影响

Effects of arsenic trioxide injection at Dazhui(GV14) on cytokines in asthmatic rats

目的观察三氧化二砷(As2O3)大椎穴注射对哮喘大鼠肺组织EOS、血清和支气管肺泡灌洗液(BALF)中IL-17、IL-4的影响。方法将大鼠随机分为对照组、哮喘组、As2O3组和地塞米松(Dx)组,每组10只。采用卵白蛋白复制大鼠哮喘模型,并给予各组相应的处理。检测各组大鼠支气管黏膜下层EOS数及气道壁EOS凋亡指数,血清和BALF中IL-4、IL-17的含量变化。结果①支气管黏膜下EOS数:哮喘组高于对照组(P<0.01);As2O3组和Dx组低于哮喘组(P<0.01),但高于对照组(P<0.01);两治疗组之间比较,差异无统计学意义(P>0.05)。②EOS凋亡指数:哮喘组明显低于对照组(P<0.01);As2O3组多于哮喘组但低于对照组,差异均有统计学意义(P<0.01);Dx组明显高于哮喘组(P<0.01)。③哮喘组BLAF中IL-4、IL-17含量明显高于对照组(P<0.01),As2O3组和Dx组血清IL-4、IL-17含量低于哮喘组(P<0.01),高于对照组(P<0.01),但两治疗组之间差异无统计学意义(P>0.05)。结论三氧化二砷大椎穴注射能抑制哮喘大鼠气道炎症。

Objective To observe the effects of arsenic trioxid injection at acupoint Dazhui（GV14） on EOS in lung tissues, and IL-17 and IL-4 in serum and branchoalvolar lavage fluid（BALF）. Methods The rats were randomized＇into four groups： control group with physiological saline, asthma group, As2O3 group and dexamethasone group, 10 rats in each group. Asthma was duplicated by ovalbumin. The EOS counts and EOS apoptosis in tracheas mucosal substratum, and the contents of IL-4 and IL-17 in serum ＆ BALF were determined. Results ① The count of EOS in asthma group obviously increased, higher than that in the control group （ P 〈 0.01 ） ; they were lower in As2 03 group and dexamethasone group （ P 〈 0.01 ） ,. but higher than the control group （ P 〈 0.01 ）, with no significance between As2O3 group and dexamethasone group （P 〉 0.05 ）. ②The EOS apoptosis in asthma group obviously decreased, lower than that in the control group （ P 〈 0.01 ） ; they were higher in As2O3 grouP and dexamethasone group （ P 〈 0.01 ）, but lower than the control group （ P 〈 0.01 ）, with no significance between As2O3 group and dexamethasone group （P 〉 0.05 ）. ③The contents of IL-4 and IL-17 in serum and BLAF were higher in asthma group than the control group （ P 〈 0.01 ） ; they were lower in As2 03 group and dexamethasone group （ P 〈 0.01 ）, but higher than the control group （ P 〈0.01 ）, with no significance between Ass 03 group and dexamethasone group （P 〉 0.05 ）. Conclusion Arsenic trioxide injection at Dazhui（GV14） can inhibit airway inflammation in asthma rats.