化学发光法快速高通量检测双嘧达莫

Detection of dipxridamole by peroxyoxalate-based high-throughput chemiluminescence

根据过氧化草酸酯与双氧水作用产生的高能中间体能激发荧光物质发光的原理,建立了利用过氧化草酸酯(TCPO)-H2O2-咪唑化学发光体系测定双嘧达莫的新方法.研究了溶剂、酸度、增敏剂及化学发光试剂浓度对化学发光信号的影响.在最佳试验条件下,双嘧达莫浓度在3.3×10-9～1.7×10-6 g/mL范围内其相对化学发光强度与浓度呈良好的线性关系,检出限为1.1×10-9 g/mL.该方法操作简便快速、灵敏度高、试剂用量少,可用于实际样品血清及尿液的检测.

Based on the fact that the bis-（2,4,6-trichlorophenyl） oxalate（TCPO）and hydrogen peroxide（H2O2）can produce a high energy intermediate to transfer its energy to fluorescent compound resulting in luminesce,a highly sensitive,simple,rapid,less consuming reagent method for the determination of dipyridamole was constructed.Optimization of the analysis conditions,including solvent,acidity,catalyst,and the concentration of CL reagents,have been investigated.This method exhibited high performance with a linear correlation between luminesce intensity and amount of dipyridamole in the range 3.3×10-9～1.7×10-6 g/mL and the detection limit of 1.1×10-9 g/mL（S/N=3）.The proposed method has been used for successful analysis of the amount of dipyridamole in serum and urine samples.