长牡蛎肉小肽营养液的研制

The Preparation of Small Peptides Nutrient Solution by Enzymatic Method from Ostrea Gigas Thunberg Meat

研究长牡蛎肉小肽营养液制备的工艺条件。先以水解度为指标,确定长牡蛎肉中性蛋白酶、木瓜蛋白酶、菠萝蛋白酶单酶的最适水解条件,通过正交试验确定各单酶水解的最佳工艺条件;然后以此为基础进行三酶复合试验,根据水解度确定最佳工艺条件,制备长牡蛎肉水解液;最后以该水解液为原料,通过正交试验确定最佳配方,按最佳配方制备小肽营养液。在最佳工艺条件下,长牡蛎肉三酶复合水解度达52.97%,经聚丙烯酰胺凝胶垂直板电泳测定,小肽峰值相对分子质量范围在300～1 000之间;17种游离氨基酸含量500.16 mg/100 mL;小肽营养液最佳配方:水解液(mL)∶蔗糖(g)∶柠檬酸(g)∶氯化钠(g)为100∶10∶0.2∶0.2,游离氨基酸总量≥5 000mg/L。本小肽营养液营养丰富,制备工艺较简单,可应用于长牡蛎肉的高值化开发。

To study the technological conditions of small peptides nutrient solution preparation from Ostrea gigas Thunberg meat. first ,Taking the hydrolyzing degree as the target to determine the optimum conditions of Ostrea gigas Thunberg meat hydrolyzed respectively by neutral protease, papain and bromelain, based on orthogonal test, the best technological conditions hydrolyzed respectively by single protease were confirmed; and then three enzymes composite en- zyme hydrolysis tests were proceeded.on the basis of hydrolyzing degree ,the perfect match and the best technological conditions of enzyme hydrolysis were confirmed. On these grounds, the enzyme hydrolysate of Ostrea gigas Thunberg meat was prepared. Finally, small peptides nutrient solution was made from the enzyme hydrolysate under the condition of optimum formula confirmed by the orthogonal test.the composite hydrolyzing degree of Ostrea gigas Thunberg meat hy- drolyzed by three protease can reach 52,97% under the optimum technological conditions, The peak value of relative molecular mass of these small peptide varied from 300 to 1 000 determined by polyacrylamide gradient gel vertical fiatbed electrophoresis, the content of 17 free amino acids was 500A6 rag/100 mL. The optimum formula of small peptides nutrient solution. ：hydrolysate （mL） ： sucrose （g） ： citric acid （g） ： sodium chloride （g）in proper sequence were as follow 100：10： 0.2：0.2. The total amount of free amino acids≥5 000 mg/L. The nutritious liquid with eutrophy and simple preparation technology can be be applied to the high-priced exploitation of Ostrea gigas Thunberg meat.