越橘抗紫外线和微波辐射作用的研究

The Research on the Anti-ultraviolet and Microwave Radiation Effect of Billberry

目的:探讨越橘浸提液在不同条件下对酵母防紫外线或微波辐射作用效果。方法:采用亚甲基蓝染色法鉴别酵母细胞是否存活,通过检测4组酵母被紫外线UVC或微波照射后存活率来衡量越橘浸提液对酵母的防辐射效果。试验分为蓝莓浸提液辐射组、红豆浸提液辐射组、生理盐水辐射组、辐射对照组。结果:随着照射时间的缩短,照射距离的延长,越橘浸提液浓度增加,对酵母细胞防紫外辐射作用增强。在照射时间1 h,照射距离为53 cm,质量浓度大于等于0.06 g/mL时越橘浸提液有较好的防紫外线辐射功效。在条件变化时,蓝莓浸提液比红豆浸提液稳定性强。在低功率(100 W)微波下照射时间20 s时,两种越橘浸提液的防辐射能力差不多,均在80%左右;功率400 W时蓝莓浸提液防辐射能力仍有65%以上,而红豆浸提液减弱幅度较大;辐射功率为800W时,覆盖蓝莓提取液的细胞依然还有50%的存活率,红豆只有20%左右。结论:蓝莓和红豆浸提液具有较强的防紫外线(UVC)和微波辐射作用。

Objective： Inquire into the anti-ultraviolet and microwave radiation effect of the blueberry extract on the yeast under different conditions. Method：the methylene blue staining method was used to distinguish the living and dead cell, useing the cell viability which was measured through four groups beening exposured by UVC or microwave to judge the radiation proof effect of the blueberry extract on the yeast. The experiment was divided into four groups：blueberry radiation, vaccinium vitis-idaea extract radiation, physiological saline radiation and control group. Result：the radiation proof effect of the billberry on the yeast was stronger as the irradiation time was shorter, the irradiation distance was longer and concentration of the billberry extract was increasing. When the irradiation time was 1 h, irradiation distance was 53 cm,the concentrations〉0.06 g/mL, the effect was better, when the conditions changes, the stability of the blueberry was stronger than vaccinium vitis-idaea. When the power of the microwave was low at 100 W and the time 20 s, both two kinds of blue berry had the same radiation proof result 80%. When the power was at 400 W, the radiation proof effect of the blueberry was still more than 65% and the other one was weaker. When the power was high at 800 W, 50% of the cell over the blueberry extract was still live, but the vaccinium vitis-idaea was only about 20%. Conclusion：both blueberry and vaccinium vitis-idaea has the radiation proof action of anti-UVC and mirowave.