Bcl-2表达与小鼠腹腔巨噬细胞凋亡的关系

The Relationship between Bcl-2 Expression and Apoptosis of Murine Peritoneal Macropgages

利用免疫荧光技术和激光扫描共聚焦显微镜（共焦镜）技术检测了地塞米松介导小鼠腹腔巨噬细胞凋亡时凋亡抑制基因 Ｂｃｌ－２表达的时空变化。结果显示，免疫荧光技术和共焦镜技术可对细胞内的蛋白表达作定量和定位检测，凋亡巨噬细胞内 Ｂｃｌ－２ 逐渐减少；核中 Ｂｃｌ－２相对量逐渐减少，胞浆中 Ｂｃｌ－２相对量逐渐增多；Ｂｃｌ－２表达和地塞米松介导的巨噬细胞凋亡是极显著负相关。结果表明，地塞米松介导巨噬细胞凋亡时，凋亡抑制基因Ｂｃｌ－２表达发生时空改变，Ｂｃｌ－２表达极显著抑制地塞米松介导巨噬细胞凋亡。免疫荧光技术结合共焦镜技术是一种理想的蛋白表达检测方法。

By combined using of the immunofluorescence and laser scanning confocal microscope, the space-time changes in expression of apoptosis-inhibited gene Bcl-2 in apoptotic macrophages induced by dexamethasone were examined. The results showed, the intracellular protein expression can be detected quantitatively and orientationally. Bcl-2 in the apoptotic cells decreased gradually. The relative quantities of tile Bcl-2 in the nucleus of apoptotic cells decreased also. But that in the cytoplasm increased. There existed an apparent significant negative correlation between expression of Bcl-2 and macrophage apoptosis. The results indicated, there occurred space-time changes in expression of apoptosis-inhibited gene Bcl-2 in apoptotic macrophages induced by dexamethasone. The macrophage apoptosis was inhibited markedly by Bcl-2. Combined using of the immunofluorescence and laser scanning confocal microscope was an ideal method for detection of intracellular protein expression.