响应面优化紫萍黄酮提取工艺及其抗氧化活性

Response surface methodology for optimization of extraction flavonoids from Spirodela polyrrhiza(L.) Schleid and antioxidant evaluation

采用响应面法优化微波提取紫萍黄酮的条件。在单因素实验的基础上,选取乙醇体积分数、微波功率、液料比为影响因子,应用Box-Behnken中心组合法进行三因素三水平的试验设计,以紫萍黄酮得率为响应值,进行响应面分析(RSM),结果表明:微波提取紫萍黄酮的最佳提取条件为乙醇体积分数62%、液料比17∶1(mL/g)、微波功率500 W、微波时间9 min;在优化工艺下,紫萍黄酮的得率达到4.14%。对微波提取的紫萍黄酮进行抗氧化性研究发现紫萍黄酮对DPPH.清除作用较好,其抗氧化活性要优于相同浓度的抗坏血酸(VC)和2,6-二叔丁基对甲酚(BHT)。

Response surface methodology was used to optimize microwave-assisted extraction of flavonoids from Spirodela polyrrhiza（L.） Schleid.Based on single factor tests,Box-Behnken central composite experiment design was carried out with three correlative factors,i.e.,solid-liquid ratio,ethanol concentration and microwave power.Response surface analysis was used to determine the effect of prime factors on the yield of flavonoids from Spirodela polyrrhiza（L.） Schleid.Results showed that the yield of flavonoids from Spirodela polyrrhiza（L.） Schleid was 4.14%.Under the optimal condition,the solid-liquid ratio was 1∶17,the ethanol concentration was 62% and the microwave power was 500 W for 9 min.Antioxidant activities were evaluated by using 1,1-diphenyl-2-picrylhydrazyl（DPPH） radical in vitro.The result showed that the DPPH radical scavenging activity of the flavonoids was stronger than that of vitamin C and 2,6-di-tert-butyl-4-methyl phenol on the same concentration.