延胡索乙素对老年大鼠红细胞膜影响的实验研究

The Effect of dl-THP on Red Cell Membrane among Aged Rats

目的:从红细胞膜成分变化角度研究延胡索乙素改善老龄大鼠血液流变学的机制。方法:将老龄Wistar大鼠随机分为老龄空白组、老龄延胡索乙素高、低剂量组、阿司匹林组,并以青年Wistar大鼠作为对照组,灌胃14天后取血、制膜,测红细胞膜上胆固醇、唾液酸、巯基、MDA含量以及SOD活性、Na+-K+-ATP酶活性。结果:延胡索乙素高剂量可明显增强老龄大鼠红细胞膜Na+-K+-ATP酶活性(P<0.05);降低红细胞膜胆固醇(P<0.01);升高膜上巯基含量(P<0.01);明显提高红细胞膜SOD活性(P<0.05);降低膜上MDA水平(P<0.05)。结论:延胡索乙素改善老龄血瘀模型大鼠血液流变学的机制可能与提高Na+-K+-ATP活性、降低膜胆固醇含量、升高红细胞膜表面巯基含量、提高膜SOD活性、降低MDA含量有关。

Objectives： To study the mechanism of dl- tetrahydropalmatine（ dl- THP） on the whole- blood viscosity at low , medium and high shear, the blood plasma viscosity and erythrocyte deformability in aged rats. Methods： Totally 40 aged Wistar rats were randomly divided into four groups： the aged controlled group, the dl - THP high dosage group, the dl - THP low dosage group, and Asprine group, of 10 rats in each group. The other 10 young rats were the young controlled group. After 14 days of intragastric administration all the rats were draw blood from carotid artery and prepared red cell membrane, and then we determined and ana- lyzed the content of Cholesterol, Saliva acid, Sulphur, MDA and activity of SOD and Na ＋ - K ＋ - ATP en- zymes. Results： High dosage of dl- THP（132.8mg/kg/d） could enhance the activity of Na~ -K~ -ATP enzymes and SOD on the red cell membrane of aged rats （ P 〈 0. 05 ） , reduce the Cholesterol content （ P 〈 0. 01 ）and MDA（P 〈0.05） content on the red cell membrane of aged rats, and could increase the Sulphur content on the erythrocyte membrane of the aged rats（P 〈 0. 01 ） . Cunclusions： The mechanism of dl - tetrahydropaimatine（ dl-THP） on the whole -blood viscosity at low ,medium and high shear, the blood plasma viscosity and erythrocyte deformability in aged rats may be related to that dl -THP ,which can enhance the activity of Na ＋ - K ＋ - ATP enzymes, reduce the Cholesterol content, increase the Sulphur content, and en- hance the SOD activity and decrease MDA content.