系统性红斑狼疮患者血清sCD14测定

Detection of Soluble CD14 in Serum of Patients with Systemic Lupus Erythematosus

目的 通过检测 SLE患者血清 sCD14水平，研究其与 SLE有关指标的关系，以探讨 CD14在 SLE发病中的作用。方法 收集 SLE患者和正常人群血清，用双抗体夹心 ELISA法测 A值，在标准曲线上查 sCD14含量。 结果 94例 SLE患者血清 sCD14较正常对照人群显著增高，且活动期高于缓解期，与疾病活动指数、 ANA滴度呈显著正相关，与 C3呈显著负相关。狼疮带试验阳性组与阴性组比较，前者明显高于后者。结论 SLE患者血清 sCD14水平对 SLE诊断和随访可能起到一定的临床提示作用。

Objective To study the correlation between serum level of soluble CD14(sCD14) and the activity parameters of systemic lupus erythematosus(SLE). Methods Ninety four SLE cases were randomly selected from our outpatients and inpatients. Sixty six healthy controls were studied in parallel. Enzyme linked immunosorbent assay(ELISA) was performed to measure the serum sCD14 levels. Results Increased serum sCD14 levels were observed in all SLE patients, compared with those of normal controls. Higher serum concentrations of sCD14 were found in active SLE patients than those under remission. A significant positive correlation between serum sCD14 levels and visceral damage as well as ANA titres was observed, and a significant negative correlation between serum sCD14 level and C3 concentrations was observed. Whereas, the correlations between serum sCD14 level and anti dsDNA antibody level, between sCD14 level and immunoglobulin (IgG,IgM, IgA) level were not found. Conclusions The detection of serum sCD14 level is useful for the diagnosis and follow up of SLE cases.