肝门阻断后细菌移位和门静脉血中内毒素变化的研究

Intestinal bacterial translocation and change in endotoxin level of portal vein blood after hepatic porta blocking

目的 研究肝门部血流阻断后肠粘膜损害所致细菌移位及门静脉血中内毒素的变化。方法 采用兔动物模型,在无菌条件下机械性阻断肝十二指肠韧带,观察阻断后15,20,30min和60min时门静脉血中内毒素值和肠系膜淋巴结细菌培养阳性数。结果 实验组肝门阻断后门静脉血内毒素值和细菌培养阳性数明显高于对照组(P<0.01),且随阻断时间延长加重。结论 缺血、缺氧是肠粘膜屏障损害的直接原因,深入对肠粘膜屏障功能保护的研究将有利于指导临床工作。

Objective Abstract: Objective To study the intestinal bacterial translocation and changs in endotoxin level of portal vein blood after hepatic inflow blocking. Methods Under aseptic condition, mechanically blocking of the hepatoduodenal ligement was adopted in rabbit model, both endotoxin level of the portal blood and positive bacterial cultures in the mensenteric lymph nodes were observed at 15, 20, 30 and 60 min respectively after the blocking. Results Both endotoxin level and positive bacterial cultures increased significantly (P<0.01) in the blocking group, which was time dependent. Conclusions Ischemia and anoxia are the direct causes of intestinal mucosa barrier damage, and a further study on the protection of the intestinal mucosa barrier could benefilial of clinical care.