白藜芦醇对兔动脉粥样硬化肢体缺血再灌注损伤的影响

Effects of resveratrol on ischemia-reperfusion injury in limbs of atherosclerosis in rabbits

目的:探讨白藜芦醇对兔动脉粥样硬化肢体缺血再灌注损伤的影响,评价其对兔肢体缺血再灌注损伤的保护作用。方法:新西兰白兔12只,高脂饮食后随机分为假手术组,缺血再灌注损伤组,白藜芦醇治疗组。缺血再灌注损伤组微血管夹夹闭股动脉5 h后恢复血流,白藜芦醇治疗组予10 mg/(kg.d)剂量治疗。观察不同组间血清磷酸肌酸激酶(CK)、乳酸脱氢酶(LDH),血清和后肢肌肉中丙二醛(MDA),超氧化物歧化酶(SOD)的差别。结果:与缺血再灌注损伤组相比,白藜芦醇治疗组术后第7天、第14天CK、LDH降低,SOD升高(P<0.05)。术后第7天MDA较缺血再灌注损伤组降低(P<0.05)。结论:白藜芦醇能改善兔后肢缺血再灌注损伤后的部分血清学指标,减轻缺血再灌注时肌细胞损伤的程度。

Objective To investigate the effects of resveratrol on ischemia -repeffusion injury in limbs of atherosclerosis in rabbits, and to assess the protection of resveratrol for ischemia -reperfusion injury. Method After high -fat diet, twelve healthy rabbits were divided in- to sham operation group, resveratrol group and ischemia- reperfusion injury group. Resveratrol [ 10 mg/（ kg . d）] was injected,5 h later ischemia followed by reperfusion in resveratrol group and no resveratrol was used in ischemia - reperfusion injury group. The contents of CK,LDH,MDA and SOD in the serum and MDA, SOD in the muscle were measured respectively and the difference was studied. Results CK, LDtt in the serum were significantly lower and SOD was significantly higher in ischemia - repeffusion injury group than that in resveratrol group （ P 〈 0. 05 ）. Conclusion Resveratrol can reduce the extent of damage caused by isehemia - reperfusion injury.