胎鼠宫内窘迫后脑组织的超氧化物歧化酶、丙二醛及一氧化氮的变化

Changes of Cerebral Superoxide Dismutase, Malondialdehyde and Nitric Oxide after Intrauterine Fetal Distress in Rats

目的 观察胎鼠宫内窘迫后脑组织超氧化物歧化酶 (SOD)、丙二醛 (MDA)、以及一氧化氮 (N O)的变化 ,以探讨新生儿期的缺血缺氧性脑损伤 (HIBD)是否在宫内已经开始发生。 方法 本研究通过钳夹子宫动静脉、卵巢动静脉造成胎鼠宫内窘迫模型。其中 A组胎鼠 13只 ,B组 10只 ,C组 11只 ,分别为钳夹导致缺血 5、10和 2 0 m in。对照组 1为假手术组 ,有胎鼠 13只。E组胎鼠 11只 ,F组 8只 ,分别为缺血 10 m in后再灌注 5 min及 2 0 min。对照组 2为单纯缺血 10 m in组 ,有胎鼠 10只。以比色法测定脑组织匀浆后的 SOD、MDA及 NO。 结果 与假手术组相比 ,缺血 10、2 0 m inSOD降低显著 ,NO升高显著 (P值分别为 <0 .0 5、<0 .0 1) ,MDA仅缺血 2 0 min才显著升高 (P<0 .0 5 )。与单纯缺血相比 ,缺血 10 m in后再灌注 5 m in SOD、MDA及 NO均未发生显著变化 ;而灌注 2 0m in三者变化均显著 (P值分别为 <0 .0 1、<0 .0 5、<0 .0 1)。 结论 重度宫内窘迫后 ,脑组织出现的SOD、MDA及 NO变化 ,与新生儿 HIBD中所见相一致 ,说明重度窘迫后的 HIBD可能在宫内已经开始发生 ,并随血供的恢复而进行性加重。

Objective To investigate the changes of cerebral superoxide dismutase(SOD), malondialdehyde(MDA)and nitric oxide(NO) after intrauterine fetal distress in rats in order to defermine whether hypoxic ischemic brain damage(HIBD) occurred in uterus. Methods Fetal distress model was made by clamping the vessels supplying to fetal rats. Group A(13 pups)was clamped for 5 minutes, group B(10 pups) and C (11 pups) was for 10 and 20 minutes respectively. Group D(13 pups) worked as control 1. After clamping for 10 minutes, Group E (11 pups) was reperfused for 5 minutes, and F(8 pups)for 20 minutes, Group G(10 pups) with no reperfusion as control 2. The level of SOD, MDA and NO in cerebral tissues were measured. Results The activity of SOD in group B and C decreased significantly than that of control 1( P <0.05 , <0.01,). MDA in group C, NO in group B and C increased significantly ( P <0.05,<0.05,<0.01 respectively). Among reperfusion groups, SOD, MDA and NO changed greatly only in group F ( P <0.01,<0.05,<0.01). Conclusion Changes of cerebral SOD, MDA and NO after severe intrauterine fetal distress is similar to that of being seen in HIBD. So the newborn's HIBD might have started in uterus after severe fetal distress.