缺氧缺血性脑损伤后脑内转化生长因子基因转录的研究

Gene Transcription of Transforming Growth Factor in Brain after Hypoxic Ischemic Brain Damage (HIBD)

目的 研究转化生长因子 (TGF) - β1 在缺氧缺血性脑损伤 (HIBD)后脑内基因转录的变化。 方法 制备左脑 HIBD的新生大鼠模型。利用反转录聚合酶链反应 (RT- PCR)和 DNA- RNA斑点杂交技术检测新生大鼠 H IBD后不同时间海马、皮质 TGF-β1 基因转录水平的表达 ,经凝胶成像及分析系统扫描 RT- PCR扩增产物 ,以内参照甘油醛 - 3-磷酸脱氢酶 (GAPDH)半定量分析 TGF- β1m RNA的动态变化。 结果 (1) RT- PCR和斑点杂交技术最低可分别检出 10 0 fg、lpg总 RNA。 (2 )HIBD后 48h TGF- β1 m RNA出现 ,72 h为表达高峰 ,5 d后基本消失 ,高峰表达时左脑 TGF- β1 m RNA水平为右脑 2 .7倍 (P<0 .0 1) ,海马表达略高于皮质。 结论 TGF-β1 转录水平表达在 HIBD后稍晚期出现 ,其作用可能与损伤修复有关。

Objective To study gene transcription of transforming growth factor(TGF)β 1 in brain after HIBD. Methods A model of HIBD in newborn rats was established.TGF β 1 mRNA in the lesioned ischemic cortex and hippocampus was monitored on different times after HIBD by reverse transcription polymerase chain reaction(RT PCR) and DNA RNA dot blot hybridization. Semiquantitative analysis of RT PCR product of TGF β 1 was done with internal control of glyceraldehyde 3 phosphate dehydrogenase (GAPDH) by White/UV Transilluminator. Results 1.At least 100 fg,lpg total RNA can be detected by RT PCR and dot blot hybridization.2.TGF β 1 mRNA was appeared at 48h after HIBD in both cortex and hippocampus and dissappeared after 5 days. Peaking transcrption was shown on 72 h when TGF β 1 mRNA transcription in the left was 2.7 times greater than the right ( P<0.01 ) and TGF β 1 mRNA gene transcription in the hippocampus was a little greater than that of the cortex. Conclusion That the TGF β 1 transcription later after HIBD may play important roles in the repairement of HIBD.