脂联素及其受体2mRNA在非酒精性脂肪性肝炎大鼠的表达及替米沙坦的干预作用

The expression of adiponectin and adiponectin receptor R2 mRNA in rats with nonalcoholic steatohepatitis and the intervention effects of telmisartan on it

目的探讨替米沙坦对非酒精性脂肪性肝炎(NASH)大鼠肝组织脂联素及其受体R2mRNA表达的影响。方法 35只雄性SD大鼠随机分为正常对照组(NC组,n=10)、模型组(FC组,n=15)和替米沙坦干预组(FT组,n=10)。FC和FT组给予高脂饲料喂养16周诱发脂肪性肝炎,其中FT组于高脂喂养12周后,给予替米沙坦(5mg·kg-1·d-1)灌胃治疗4周。取肝组织测肝指数;检测血清ALT及AST,RT-PCR方法测定大鼠肝组织脂联素及其受体R2mRNA的表达。结果与NC组相比,FC组血清ALT、AST均明显升高(P<0.01),脂联素及其受体R2mRNA水平均显著降低(P<0.01);FT组的血清ALT显著降低(P<0.01),脂联素及其受体R2mRNA水平较FC组均升高(P<0.05)。结论替米沙坦对实验性非酒精性脂肪肝大鼠具有一定的治疗作用,其作用机制之一是促进肝脏脂联素及其受体R2mRNA的表达。

Objective To investigate the effects of telmisartan on the expression of adiponectin and adiponectin receptor R2 mRNA in rats with nonalcoholic steatohepatitis （NASH）. Methods 35 male SD rats were randomly divided into normal control group（NC group, n = 10）,NASH model group（ FC group, n = 15） and telmisartan intervention group（ FT group, n = 10）. The rats in NC group were given standard diet,however,the rats in the other two groups were given high fat diet for 16 weeks to induce NASH. After 12 weeks the rats in FT group were given telmisartan （Stag · kg-1 · d-1 ） for 4 weeks by gavage. At the end of the 16th week,all the animals were sacrificed and the rat＇s body weight was recorded,liver indexes were determined,the levels of ALT and AST in serum were detected by using automatic biochemistry analyzer, and the expressions of adiponeetin and adiponectin receptor 1t2 mRNA in liver tissue were detected by RT-PCR. Results As compared with those in NC group,the levels of serum ALT and AST were significantly increased in FC group （ P 〈 0.01 ）, however, the expressions of adiponectin and adiponectin receptor R2 mRNA in liver tissue were significantly decreased in FC group （ P 〈0.01 ）. As compared with those in FC group,the levels of serum ALT was obviously decreased in Ft group （ P 〈0.01 ）, however, the levels of adiponectin and adiponectin receptor R2 mRNA in liver tissue were significantly higher than those in FT group （ P 〈 0.05）. Conclusion Telmisartan is effective in treating rats with NASH,and one of the action mechanisms is that telmisartan can promote the expression of adiponectin and adiponectin receptor R2 mRNA in liver.