β肽防治肝癌转移复发的研究

An experimental study of the effect of β peptide on liver cancer recurrence and metastasis

目的 观察 β肽对SMMC 772 1肝癌细胞株侵袭粘附能力和对裸鼠移植人肝癌切除术后转移复发的影响。方法 以细胞侵袭实验观察β肽对SMMC 772 1细胞侵袭运动能力及对已与FN粘附的 772 1细胞脱落的影响。以MTT法测量 β肽对 772 1细胞与FN(fibronectin)粘附的影响。以细胞粘附实验观察 β肽对 772 1 772 1细胞 ,772 1 内皮细胞之间粘附的影响。以LCI D2 0裸鼠人肝癌转移模型为材料 ,观察 β肽对早期和晚期肝癌切除术后癌转移复发的影响。 结果 772 1细胞膜ICAM 1(inter cellularadhesionmolecule 1)表达对照组为 15 31± 2 0 9,β肽组为 5 71± 14(P <0 0 1)。实验中止时 772 1细胞粘附于FN的细胞OD值为阳性对照组 5 7± 9,β肽组 13± 9(P <0 0 5 )。 772 1 772 1,772 1 内皮细胞粘附率在实验中止时分别为对照组 (6 0 8± 10 1) %、(96 2± 11 1) % ,β肽组〔(15 9± 2 5 ) % ,P <0 0 1〕、〔(2 2 6± 9 1) % ,P <0 0 1〕。动物实验 :肝内癌转移灶数目、癌转移灶累及肝叶数、切缘复发癌直径分别为 :早期肝癌切除术后对照组 7 2± 2 3、3 7± 0 6、(1 1± 0 2 )cm ,β肽组 0 0± 0 0 (P <0 0 1)、0 0±0 0 (P <0 0 1)、〔(0 9± 0 6 )cm ,P >0 0 5〕 ;晚期肝癌切除术后对照组 8

Objectives[WT5”BZ] To study the inhibition effect of β peptide on adhesion and invasion ability of SMMC 7721 (HCC) cell line and the prevention of β peptide on liver cancer recurrence and metastasis after hepatectomy in a nude mouse model.[WT5”HZ] Methods[WT5”BZ] Analyzing the blocking effect of β peptide on 7721 cell membrane ICAM 1 by immunofluorescence flow cytometry to observe the influence of β peptide on invasion ability of 7721 cell by cell migration experiment and the detachment of attached 7721 cell, the influence of β peptide on 7721 cell FN adhesion by MTT method, the adhesion of 7721 7721 cell, 7721 endothelial cell by cell adhesion experiment. β peptide was injected introabdominally to observe its effect on the recurrence and metastasis of nude mice bearing human liver cancer after hepatectomy.[WT5”HZ] Results[WT5”BZ] Cells incubated with β peptide had lower ICAM 1 expression than that without the treatment (571±14 vs 1531±209, P <0 01). The OD (optical density) of 7721 cell adhesion to FN at the end of experiment were 56 6±9 0 in the control group and 13 4±8 7( P <0 01) in the β peptide group. The adhesive rates of 7721 7721, 7721 endothelial cell were 60 8±10 1, 96 2±11 1 in the control group and 15 9±2 5 ( P <0 01), 22 6±9 1 ( P <0 01) in the β peptide group respectively at the end of experiment. Intrahepatic metastasis nodes, metastasis involved hepatic lobe and tumor diameter were 7 2±2 3, 3 7±0 6, 1 1±0 2 in the control group and 0 0±0 0 ( P <0 01), 0 0±0 0( P <0 01), 0 9±0 6( P >0 05) in the β peptide group respectively after early hepatectomy;while at advanced stage undergoing hepatectomy,there were 8 6±8 2, 4 6±1 5, 0 9±0 4 in the control group and 4 2±5 0 ( P >0 05), 2 2±1 3( P <0 05), 0 7±0 1( P >0 05) in the β peptide group respectively. Lung metastatic nodes and metastasis involved organs were 2 8±2 5, 6 8±0 5 in the control group and 0 0±0 0( P <0 05), 1 0±1 7( P <0 01)in the β peptide group after early hepatectomy, and 63 8±28 1, 6 8±3 0 in the control group and 6 8±11 9 ( P <0 05), 3 8±0 8( P <0 05)in the β peptide group after hepatectomy at advanced stage. [WT5”HZ]Conclusions[WT5”BZ] β peptide could inhibit the invasion and adhesion of SMMC 7721 cell, and could also prevent and inhibit liver cancer metastasis and recurrence after hepatectomy in a nude mouse model.