普罗布考、厄贝沙坦对自发性高血压大鼠糖代谢及血清脂联素的影响

Effect of probucol and irbesartan,alone and in combination,on glycometabolism and serum adiponectin of spontaneously hypertensive rat

目的探讨抗氧化剂普罗布考、血管紧张素Ⅱ1型受体拮抗剂(AT1R)厄贝沙坦对自发性高血压大鼠(SHR)胰岛素抵抗及血清脂联素的影响。方法 SHR40只随机分为4组,对照组(n=10)、普罗布考组[61mg/(kg.d),n=10]、厄贝沙坦组[50mg/(kg.d),n=10]、联合用药组(普罗布考+厄贝沙坦,n=10);另设同种系雄性WKY大鼠为对照(n=5)。采用灌胃法给药12周,给药结束后,用生化方法测定糖代谢指标,ELISA方法测定血清脂联素含量。结果 SHR和WKY大鼠空腹血糖水平未见明显差异,SHR空腹胰岛素水平高于WKY组[对照组(15.03±3.72)比(11.31±2.53)mU/L;普罗布考组(16.40±3.64)比(11.31±2.53)mU/L;厄贝沙坦组(15.09±3.08)比(11.31±2.53)mU/L;联合用药组(14.34±3.07)比(11.31±2.53)mU/L),均P<0.05]。SHR之间空腹胰岛素水平差异无统计学意义;OGTT结果显示普罗布考组及对照组大鼠30min血糖水平[(12.23±0.71)比(10.52±0.87)mmol/L;(12.57±1.00)比(10.52±0.87)mmol/L,均P<0.01]、120min血糖水平[(8.72±1.18)比(7.28±0.89)mmol/L;(8.78±1.29)比(7.28±0.89)mmol/L,均P<0.01]高于厄贝沙坦组;普罗布考组、厄贝沙坦组及联合用药组脂联素水平高于对照组[(36.58±10.84)比(22.42±2.55)mg/L;(37.54±11.27)比(22.42±2.55)mg/L;(38.47±2.86)比(22.42±2.55)mg/L,均P<0.05]。结论普罗布考及厄贝沙坦可升高SHR血清脂联素水平,厄贝沙坦可降低SHR餐后30、120min血糖水平。

Objective To investigate the effect of probucol and irbesartan on insulin resistance and serum adiponectin of spontaneously hypertensive rat（SHR）.Methods Fourty SHR were divided into four groups and treated with placebo（SHR-C group）,probucol[61mg/（kg.d）（SHR-pro group）,irbesartan[50mg/（kg.d）]（SHR-Irb group）,or both drugs（SHR-Pro＋Irb group）by gavaging for 12weeks.Glycometabolism were measured by biochemistry methods.Serum insulin level were measured by radio-immunity methods.Change of serum adiponectin were measured by ELISA.Results No difference of fasting blood glucose in different groups（P0.05）.Serum insulin level of four SHR groups were higher than that of WKY group[SHR-C（15.03±3.72）vs（11.31± 2.53）mU/L;SHR-pro（16.40±3.64）vs（11.31±2.53）mU/L;SHR-Irb（15.09±3.08）vs（11.31±2.53）mU/L;HR-pro＋Irb（14.34±3.07）vs（11.31±2.53）mU/L,all P0.05].No difference of serum insulin level in the four SHR groups（P0.05）;the result of OGTT showed blood glucose level of SHR-pro,SHR-C group at 30min [（12.23±0.71）vs（10.52±0.87）mmol/L;（12.57±1.00）vs（10.52±0.87）mmol/L,all P0.01],120min [（8.72±1.18）vs（7.28±0.89）mmol/L;（8.78±1.29）vs（7.28±0.89）mmol/L,all P0.01]were higher than that of SHR-Irb group;Serum adiponectin level of SHR-Pro,SHR-Irb,SHR-pro＋Irb groups were higher than that of SHR-C group[（36.58±10.84）vs（22.42±2.55）mg/L;（37.54±11.27）vs（22.42±2.55）mg/L;（38.47±2.86） vs（22.42±2.55）mg/L,all P0.05].Conclusion Probucol and irbesartan increased serum adiponectin level of SHR,and Irbesartan decreased blood glucose level at 30and 120min.