Contrary Regulation of TIMP-1 and MMP-9 by Hepatocyte Growth Factor Antibody after Lung Injury 被引量：1

Contrary Regulation of TIMP-1 and MMP-9 by Hepatocyte Growth Factor Antibody after Lung Injury

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摘要 Objective To study the influence of hepatocyte growth factor (HGF) antibody on the lung expression level of matrix metalloproteinases-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1). Methods Thirty male Wistar rats were randomly divided into 3 groups: control group, model group, and intervention group. Endotoxin was intratracheally infused in the model and intervention groups. HGF antibody was injected in the rats of the intervention group from day 1 to day 14, while the same volume of saline was injected in the control group. The rats were sacrificed on day 28 after endotoxin treatment. The amounts of MMP-9 mRNA and TIMP-1 mRNA were measured by reverse transcription-polymerase chain reaction, and protein expression levels of MMP-9 and TIMP-1 were measured by immunohistochemistry. Results In the model group, both mRNA and protein expression levels of TIMP-1 were significantly increased, the same as MMP-9. In the intervention group, the increase of TIMP-1 was remarkably reduced compared with the model group, while the mRNA and protein expression levels of MMP-9 were still increased. Conclusion HGF activity may accelerate the repair of lung injury through contrary regulating the expression levels of TIMP-1 and MMP-9.

作者 Wei-wei Yu Qin Xia

机构地区 Institute of General Department

出处《Chinese Medical Sciences Journal》 CAS CSCD 2011年第4期216-220,共5页 中国医学科学杂志（英文版）

关键词 hepatocyte growth factor matrix metalloproteinases-9 tissue inhibitor of metalloproteinases- 1 TIMP-1 肝细胞生长因子 MMP-9 肺损伤抗体基质金属蛋白酶-9 Wistar大鼠生理盐水

分类号 Q492.6 [生物学—生理学] Q516 [生物学—生物化学]

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1Ware LB,Matthay MA.Keratinocyte and hepatocyte growth factor in the lung:roles in lung development,inflammation,and repair.Am J Physiol Lung Cell Mol Physiol 2002; 282:L924-40.
2Droppelmann CA,Gutiérrez J,Vial C,et al.Matrix metalloproteinase-2-deficient fibroblasts exhibit an alteration in the fibrotic response to connective tissue growth factor/CCN2 because of an increase in the levels of endogenous fibronectin.J Biol Chem 2009; 284:13551-61.
3Carney DE,Lutz CJ,Picone AL,et al.Matrix metalloproteinase inhibitor prevents acute lung injury after cardiopulmonary bypass.Circulation 1999; 100:400-6.
4Gibbs DF,Warner RL,Weiss SJ,et al.Characterization of matrix metalloproteinases produced by rat alveolar macrophages.Am J Respir Cell Mol Biol 1999; 20:1136-44.
5Hagiwara S,Iwasaka H,Matsumoto S,et al.Antisense oligonucleotide inhibition of heat shock protein (HSP) 47 improves bleomycin-induced pulmonary fibrosis in rats.Respir Res 2007; 8:37.
6Lianxu C,HongtiJ,Changlong Y.NF-KBp65-specific siRNA inhibits expression of genes of COX-2,NOS-2 and MMP-9in rat IL-1β-induced and TNF-o-induced chondrocytes.Osteoarthritis Cartilage 2006; 14:367-76.
7Morariu AM,Maathuis MH,Asgeirsdottir SA,et al.Acute isovolemic hemodilution triggers proinflammatory and procoagulatory endothelia microcirculation activation in vital organs:role of erythrocyte aggregation.Microcirculation 2006; 13:397-409.
8Mrozek JD,Smith KM,Bing DR,et al.Exogenous surfacrant and partial liquid ventilation:physiologic and pathologic effects.Am J Respir Crit Care Med 1997; 156:1058-65.
9Coimbra R,Melbostad H,Loomis W,et al.LPS-induced acute lung injury is attenuated by phosphodiesterase inhibition:effects on proinflammatory mediators,metalloproteinases,NF-KB,and ICAM-1 expression.J Trauma 2006; 60:115-25.
10Stuelten CH,DaCosta Byfield S,Arany PR,et al.Breast cancer cells induce stromal fibroblasts to express MMP-9via secretion of TNF-alpha and TGF-beta.J Cell Sci 2005;118:2143-53.