HBV转基因小鼠的舌色改变及血流变化

Changes in color and blood flow of the tongue of HBV transgenic mice

目的:研究HBV转基因小鼠的舌色表现和微血管血流变化.方法:10只C57BL/6J-HBV转基因小鼠和10只正常小鼠(C57BL/6J非转基因小鼠),均雌雄各半,采用小鼠舌象观察装置对小鼠舌色进行观察;使用激光多普勒血流仪进行舌和肝脏微血管血流检测;断头采血,测定血清丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)的含量;HE染色观察肝和舌组织病理变化.结果:HBV转基因小鼠中,6只小鼠舌色呈紫色,4只舌色呈暗红色,而正常小鼠的舌色均为淡红色.HBV转基因小鼠舌色色调(H值)变深,有显著性差异(P<0.01);舌色亮度(V值)变弱,有极显著性差异(P<0.001).和正常鼠相比,紫舌HBV转基因小鼠微血管血流灌注量和血流速度显著降低(0.206±0.13vs0.794±0.13;0.479±0.07vs0.331±0.04,P<0.01,P<0.001),该模型肝脏微血管血流灌注量和血流速度显著降低,HBV转基因小鼠肝脏和舌存在明显的炎性改变.结论:HBV转基因小鼠舌色以紫色多见,其形成机制与微循环障碍有关.该模型存在肝脏和舌的炎症病理,提示炎性微环境是形成其肝脏和舌微循环障碍的重要原因.

AIM： To examine the changes in color and microvascular blood flow of the tongue of HBV transgenic mice. METHODS： Ten C57BL/6J-HBV transgenic mice and 10 normal mice were used in the study. The tongue color of the mice was observed daily. The microvascular blood flow of tongue and liver were detected with a laser Doppler blood flowmeter. Blood samples were taken to measure serum alanine aminotransferase （ALT） and aspartate aminotransferase （AST）. Pathological changes in the tongue and liver were evaluated by HE staining. RESULTS： The tongue was purple in color in six HBV transgenic mice and dark red in the remaining four transgenic mice. In contrast,the tongue of normal mice was light red in color. The hue of the tongue become darker and brightness become weaker in HBV transgenic mice （0.206 ± 0.13 vs 0.794 ± 0.13; 0.479 ± 0.07 vs 0.331 ± 0.04, P 0.01, P 0.001） compared to normal mice. The microvascular blood flow perfusion and velocity in the tongue and liver in HBV transgenic mice decreased significantly compared to normal mice. Obvious inflammatory changes were observed in the liver and tongue of HBV transgenic mice. CONCLUSION： The tongue of most HBV transgenic mice became purple, whose mechanism is related with microcirculation disorder. Inflammatory changes in the liver and tongue in HBV transgenic mice are perhaps the main cause of microcirculation disorder.