H3K27me3在胃癌中的表达及其临床意义

Significance of H3K27me3 expression in gastric cancer

目的:研究H3K27me3在胃癌细胞和组织中的表达,并分析与临床病理因素的关系,探讨H3K27me3在胃癌发生发展中的作用和意义.方法:应用Western blot方法检测胃癌细胞系SGC7901、BGC823、AGS和正常胃黏膜上皮细胞GES-1中H3K27me3的表达;免疫组织化学方法检测61例胃癌组织及20例正常胃黏膜组织中H3K27me3的表达.结果:与正常胃黏膜细胞GES-1相比,H3K27me3在胃癌细胞SGC7901、BGC823、AGS中高表达;H3K27me3在胃癌组织中阳性表达率为80.3%,并与肿瘤大小、浸润深度、淋巴结转移、血管侵犯、临床分期、TNM分期有关(P=0.049,0.030,0.034,0.025,0.003,0.031),而与患者的性别、年龄、病变部位、分化程度、神经侵犯之间无相关性.结论:H3K27me3在胃癌中高表达,并与肿瘤的侵袭转移有关,可能是胃癌患者重要的预后因子.

AIM： To investigate the expression of trimethylation at lysine 27 of histone H3 （H3K27me3） in gastric cancer tissue and cell lines and to evaluate its correlation with clinicopathological parameters of gastric carcinoma. METHODS： The protein expression of H3K27me3 was detected by Western blot in gastric cancer lines SGC7901, BGC823, AGS and normal gastric mucosal epithelial cell line GES-1. Immunohistochemistry （IHC） was utilized to examine the protein expression of H3K27me3 in 61 gastric cancer specimens and 20 normal gastric epithelial specimens. RESULTS： H3K27me3 expression significantly increased in gastric cancer lines （SGC7901,BGC823, and AGS） compared to normal gastric mucosal epithelial cell line GES-1. The positive rates of H3K27me3 protein expression in gastric cancer was 80.3%. The expression levels of H3K27me3 were significantly associated with tumor size, depth of invasion, lymph node metastasis, vascular invasion, clinical stage, and T staging （P = 0.049, 0.030, 0.034, 0.025, 0.003, and 0.031, respectively）, but had no correlation with patient’s age, sex, tumor location, tumor differentiation degree, or nerve invasion. CONCLUSION： High expression of H3K27me3 correlates closely with tumor invasion and metastasis in gastric cancer and may be an important prognostic factor in patients with gastric cancer.