检测植物病毒的三种血清学方法敏感性的比较

STUDY ON COMPARISON OF THREE SEROLOGICAL METHODS FOR PLANT VIRUS DETECTION

以芋花叶病毒和豇豆花叶病毒为试验材料,进行了酶联免疫吸附试验,免疫吸附电子显微术和点免疫结合试验检测植物病毒的敏感性的研究。无论是检测感染组织粗汁液还是纯化的病毒,点免疫结合试验均优于酶联免疫吸附试验和免疫吸附电子显微术。在使用羟基吲(?)磷酸盐和氮蓝四唑为碱性磷酸酶的底物时,点免疫结合试验检测纯化的豇豆花叶病毒的可测感度为0.35ng,芋花叶病毒为0.83ng。对三种检测植物病毒的血清学方法进行了比较,并讨论了点免疫结合试验的优点。

The sensitivities of enzyme-linked immunosorbent assay (ELISA), immunosorbent electron microscopy (ISEM) and dot-immunobinding assay (DIBA) were compared on their ability to detect dasheen mosaic virus and cowpea mosaic virus. The dot-immunobinding assay was superior to ELISA and ISEM for detecting these two viruses both in purified preparation and in crude sap from infected tissues. As little as 0.83ng of DMV and 0.35 ng of CpMV. were detected, using a dot-immunobinding assay in which indoxyl phosphate and nitro blue tetrazolium were used as substrates for alkaline phosphatase. The advantage of the dot-immunobinding assay is discussed.